A comprehensive ovine model of blood transfusion

Simonova, G., Tung, J. P., Fraser, J. F., Do, H. L., Staib, A., Chew, M. S., Dunster, K. R., Glenister, K. M., Jackson, D. E. and Fung, Y. L. (2014) A comprehensive ovine model of blood transfusion. Vox Sanguinis, 106 2: 153-160. doi:10.1111/vox.12076

Author Simonova, G.
Tung, J. P.
Fraser, J. F.
Do, H. L.
Staib, A.
Chew, M. S.
Dunster, K. R.
Glenister, K. M.
Jackson, D. E.
Fung, Y. L.
Title A comprehensive ovine model of blood transfusion
Journal name Vox Sanguinis   Check publisher's open access policy
ISSN 0042-9007
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1111/vox.12076
Open Access Status Not Open Access
Volume 106
Issue 2
Start page 153
End page 160
Total pages 8
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Language eng
Formatted abstract
Background: The growing awareness of transfusion-associated morbidity and mortality necessitates investigations into the underlying mechanisms. Small animals have been the dominant transfusion model but have associated limitations. This study aimed to develop a comprehensive large animal (ovine) model of transfusion encompassing: blood collection, processing and storage, compatibility testing right through to post-transfusion outcomes.

Materials and methods: Two units of blood were collected from each of 12 adult male Merino sheep and processed into 24 ovine-packed red blood cell (PRBC) units. Baseline haematological parameters of ovine blood and PRBC cells were analysed. Biochemical changes in ovine PRBCs were characterized during the 42-day storage period. Immunological compatibility of the blood was confirmed with sera from potential recipient sheep, using a saline and albumin agglutination cross-match. Following confirmation of compatibility, each recipient sheep (n = 12) was transfused with two units of ovine PRBC.

Results: Procedures for collecting, processing, cross-matching and transfusing ovine blood were established. Although ovine red blood cells are smaller and higher in number, their mean cell haemoglobin concentration is similar to human red blood cells. Ovine PRBC showed improved storage properties in saline-adenine-glucose-mannitol (SAG-M) compared with previous human PRBC studies. Seventy-six compatibility tests were performed and 17·1% were incompatible. Only cross-match compatible ovine PRBC were transfused and no adverse reactions were observed.

Conclusion: These findings demonstrate the utility of the ovine model for future blood transfusion studies and highlight the importance of compatibility testing in animal models involving homologous transfusions.
Keyword Blood transfusion
Compatibility test
Ovine model
Storage lesion
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
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