Prognostic value of ZAP-70 expression in chronic lymphocytic leukemia as assessed by quantitative polymerase chain reaction and flow cytometry

Adams, Rebecca L. C., Cheung, Catherine, Banh, Raymond, Saal, Russell, Cross, Donna, Gill, Devinder, Self, Marlene, Klein, Kerenaftali and Mollee, Peter (2014) Prognostic value of ZAP-70 expression in chronic lymphocytic leukemia as assessed by quantitative polymerase chain reaction and flow cytometry. Cytometry Part B - Clinical Cytometry, 86 2: 80-90. doi:10.1002/cyto.b.21138


Author Adams, Rebecca L. C.
Cheung, Catherine
Banh, Raymond
Saal, Russell
Cross, Donna
Gill, Devinder
Self, Marlene
Klein, Kerenaftali
Mollee, Peter
Title Prognostic value of ZAP-70 expression in chronic lymphocytic leukemia as assessed by quantitative polymerase chain reaction and flow cytometry
Journal name Cytometry Part B - Clinical Cytometry   Check publisher's open access policy
ISSN 1552-4949
1552-4957
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1002/cyto.b.21138
Open Access Status Not yet assessed
Volume 86
Issue 2
Start page 80
End page 90
Total pages 11
Place of publication Hoboken, NJ United States
Publisher Jossey Bass, Ed. and Pub.
Language eng
Subject 1307 Cell Biology
2722 Histology
2734 Pathology and Forensic Medicine
Abstract Background Chronic lymphocytic leukemia (CLL) is a disorder in which the tempo of disease progression is highly variable, and prognostic markers that can be utilized at diagnosis are regarded as clinically important. Currently, there are several prognostic factors, such as immunoglobulin heavy chain (IgV H) mutational status, and ZAP-70 protein expression in neoplastic B-cells, that have demonstrated significant discriminative power in the prognostication of CLL. They are, however, largely unavailable in the routine diagnostic laboratory setting. Methods In this study, we characterized the IgVH status and ZAP-70 expression by molecular techniques in a cohort of 108 patients with CLL, and correlated these results with three different methods of ZAP-70 expression by flow cytometry. We then assessed the results of these methods in terms of prognostic power as characterized by time to first treatment (TTFT). Results By comparing three different flow cytometry methods using receiver-operator curve (ROC) analysis, we identified that by utilizing a corrected mean fluorescence intensity (CorrMFI) algorithm for assessing ZAP-70 expression, there was good correlation with both IgVH mutational status, and ZAP-70 expression as assessed by qPCR. We were also able to show that ZAP-70 expression, as assessed by both qPCR and the CorrMFI method, was prognostic of TTFT. Conclusions While confirmation in a larger patient cohort, with longer follow-up is required, we believe that the CorrMFI represents the most promising method currently available in a routine diagnostic setting for the assessment of ZAP-70 expression in CLL patients. © 2013 International Clinical Cytometry Society
Keyword Chronic Lymphocytic Leukemia
Flow cytometry
Mean fluorescence intensity
Polymerase chain reaction
Prognosis
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Public Health Publications
School of Medicine Publications
UQ Diamantina Institute Publications
 
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