Generation and characterization of leukemia inhibitory factor-dependent equine induced pluripotent stem cells from adult dermal fibroblasts

Whitworth, Deanne J., Ovchinnikov, Dmitry A., Sun, Jane, Fortuna, Patrick R. J. and Wolvetang, Ernst J. (2014) Generation and characterization of leukemia inhibitory factor-dependent equine induced pluripotent stem cells from adult dermal fibroblasts. Stem Cells and Development, Fast Track Articles 13: 1-9. doi:10.1089/scd.2013.0461

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Author Whitworth, Deanne J.
Ovchinnikov, Dmitry A.
Sun, Jane
Fortuna, Patrick R. J.
Wolvetang, Ernst J.
Title Generation and characterization of leukemia inhibitory factor-dependent equine induced pluripotent stem cells from adult dermal fibroblasts
Journal name Stem Cells and Development   Check publisher's open access policy
ISSN 1547-3287
1557-8534
Publication date 2014-02-20
Year available 2014
Sub-type Article (original research)
DOI 10.1089/scd.2013.0461
Open Access Status Not Open Access
Volume Fast Track Articles
Issue 13
Start page 1
End page 9
Total pages 9
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert, Publishers
Language eng
Formatted abstract
In this study we have reprogrammed dermal fibroblasts from an adult female horse into equine induced pluripotent stem cells (equiPSCs). These equiPSCs are dependent only on leukemia inhibitory factor (LIF), placing them in striking contrast to previously derived equiPSCs that have been shown to be co-dependent on both LIF and basic fibroblast growth factor (bFGF). These equiPSCs have a normal karyotype and have been maintained beyond 60 passages. They possess alkaline phosphatase activity and express eqNANOG, eqOCT4, and eqTERT mRNA. Immunocytochemistry confirmed that they produce NANOG, REX1, SSEA4, TRA1-60, and TRA1-81. While our equiPSCs are LIF dependent, bFGF co-stimulates their proliferation via the PI3K/AKT pathway. EquiPSCs lack expression of eqXIST and immunostaining for H3K27me3, suggesting that during reprogramming the inactive X chromosome has likely been reactivated to generate cells that have two active X chromosomes. EquiPSCs form embryoid bodies and in vitro teratomas that contain derivatives of all three germ layers. These LIF-dependent equiPSCs likely reflect a more naive state of pluripotency than equiPSCs that are co-dependent on both LIF and bFGF and so provide a novel resource for understanding pluripotency in the horse.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Online Ahead of Editing: February 20, 2014

 
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 07 Mar 2014, 01:31:01 EST by Dr Deanne Whitworth on behalf of School of Veterinary Science