Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens

Han, Siew Ping, Gambin, Yann, Gomez, Guillermo A., Verma, Suzie, Giles, Nichole, Michael, Magdalene, Wu, Selwin K., Guo, Zhong, Johnston, Wayne, Sierecki, Emma, Parton, Robert G., Alexandrov, Kirill and Yap, Alpha S. (2014) Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens. Journal of Biological Chemistry, 289 11: 7764-7775. doi:10.1074/jbc.M113.544478

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Author Han, Siew Ping
Gambin, Yann
Gomez, Guillermo A.
Verma, Suzie
Giles, Nichole
Michael, Magdalene
Wu, Selwin K.
Guo, Zhong
Johnston, Wayne
Sierecki, Emma
Parton, Robert G.
Alexandrov, Kirill
Yap, Alpha S.
Title Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
Publication date 2014-01-27
Year available 2014
Sub-type Article (original research)
DOI 10.1074/jbc.M113.544478
Open Access Status File (Publisher version)
Volume 289
Issue 11
Start page 7764
End page 7775
Total pages 12
Place of publication Bethesda, MD, United States
Publisher American Society for Biochemistry and Molecular Biology
Language eng
Abstract Cadherin junctions arise from the integrated action of cell adhesion, signaling and the cytoskeleton. At the zonula adherens (ZA)1, a WAVE2-Arp2/3 actin nucleation apparatus is necessary for junctional tension and integrity. But how this is coordinated with cadherin adhesion is not known. We now identify cortactin as a key scaffold for actin regulation at the ZA, that localizes to the ZA through influences from both E-cadherin and N-WASP. Using cell-free protein expression and fluorescent single molecule coincidence assays we demonstrate that cortactin binds directly to the cadherin cytoplasmic tail. However, its concentration with cadherin at the apical ZA also requires N-WASP. Cortactin is known to bind Arp2/3 directly (1). We further show that cortactin can directly bind WAVE2, as well as Arp2/3, and both these interactions are necessary for actin assembly at the ZA. We propose that cortactin serves as a platform that integrates regulators of junctional actin assembly at the ZA.
Keyword Cadherin
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 631377
Institutional Status UQ
Additional Notes Published online 27 January 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 28 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 27 times in Scopus Article | Citations
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Created: Wed, 05 Mar 2014, 01:37:29 EST by Susan Allen on behalf of Institute for Molecular Bioscience