Gateway-assisted vector construction to facilitate expression of foreign proteins in the chloroplast of single celled algae

Oey, Melanie, Ross, Ian L. and Hankamer, Ben (2014) Gateway-assisted vector construction to facilitate expression of foreign proteins in the chloroplast of single celled algae. PloS One, 9 2: . doi:10.1371/journal.pone.0086841


Author Oey, Melanie
Ross, Ian L.
Hankamer, Ben
Title Gateway-assisted vector construction to facilitate expression of foreign proteins in the chloroplast of single celled algae
Journal name PloS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-02-11
Year available 2014
Sub-type Article (original research)
DOI 10.1371/journal.pone.0086841
Open Access Status DOI
Volume 9
Issue 2
Total pages 4
Place of publication San Francisco, United States
Publisher Public Library of Science
Language eng
Formatted abstract
With a rising world population, demand will increase for food, energy and high value products. Renewable production systems, including photosynthetic microalgal biotechnologies, can produce biomass for foods, fuels and chemical feedstocks and in parallel allow the production of high value protein products, including recombinant proteins. Such high value recombinant proteins offer important economic benefits during startup of industrial scale algal biomass and biofuel production systems, but the limited markets for individual recombinant proteins will require a high throughput pipeline for cloning and expression in microalgae, which is currently lacking, since genetic engineering of microalgae is currently complex and laborious. We have introduced the recombination based Gateway® system into the construction process of chloroplast transformation vectors for microalgae. This simplifies the vector construction and allows easy, fast and flexible vector design for the high efficiency protein production in microalgae, a key step in developing such expression pipelines.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article # e86841

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
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Created: Wed, 05 Mar 2014, 01:22:26 EST by Susan Allen on behalf of Institute for Molecular Bioscience