TLC screening of thraustochytrid strains for squalene production

Nakazawa, Atsushi, Kokubun, Yume, Matsuura, Hiroshi, Yonezawa, Natsuki, Kose, Ryoji, Yoshida, Masaki, Tanabe, Yuuhiko, Kusuda, Emi, Van Thang, Duong, Ueda, Mayumi, Honda, Daiske, Mahakhant, Aparat, Kaya, Kunimitsu and Watanabe, Makoto M. (2014) TLC screening of thraustochytrid strains for squalene production. Journal of Applied Phycology, 26 1: 29-41. doi:10.1007/s10811-013-0080-x


Author Nakazawa, Atsushi
Kokubun, Yume
Matsuura, Hiroshi
Yonezawa, Natsuki
Kose, Ryoji
Yoshida, Masaki
Tanabe, Yuuhiko
Kusuda, Emi
Van Thang, Duong
Ueda, Mayumi
Honda, Daiske
Mahakhant, Aparat
Kaya, Kunimitsu
Watanabe, Makoto M.
Title TLC screening of thraustochytrid strains for squalene production
Journal name Journal of Applied Phycology   Check publisher's open access policy
ISSN 0921-8971
1573-5176
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1007/s10811-013-0080-x
Volume 26
Issue 1
Start page 29
End page 41
Total pages 13
Place of publication Dordrecht, Netherlands
Publisher Springer
Abstract Screenings of thraustochytrids (Labyrinthulomycetes) have been conducted for 176 strains isolated from various sites in the Asian region to investigate what type of species and strains accumulate high levels of squalene. Thin layer chromatography (TLC) screening for squalene production revealed that 38 strains were rated as "+" (high), 29 as "±" (medium), and 109 as "-" (low). Further, high performance liquid chromatography analysis strongly supported the TLC screening results. Besides the 18W-13a strain of Aurantiochytrium sp., which was previously recognized as a squalene-rich strain, several strains produced squalene at approximately 1 g L-1 of culture volume. Squalene production was strongly related to locality, colony color, and phylogenetic clade. Most strains with "+" squalene spots were isolated from Okinawa, a subtropical region of Japan, while the strains with "±" and "-" squalene spots were isolated from wide geographical regions from tropical to subarctic. Approximately half the strains with orange colonies on GTY medium plates produced a high amount of squalene, whereas the other strains with different colors showed less or no squalene spots on TLC. All the squalene-rich strains were assigned to the Aurantiochytrium clade. Overall, our results suggest that (1) the thraustochytrids show tendentious locality in terms of squalene production, (2) a relationship exists between the metabolic synthesis of carotenoid pigments and squalene production, and (3) the Aurantiochytrium clade may have evolved to accumulate squalene.
Keyword Aurantiochytrium
Squalene
Thraustochytrid
TLC
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2014 Collection
 
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