A comparative study: The impact of different lipid extraction methods on current microalgal lipid research

Li, Yan, Ghasemi Naghdi, Forough, Garg, Sourabh, Adarme-Vega, Tania Catalina, Thurecht, Kristofer J., Ghafor, Wael Abdul, Tannock, Simon and Schenk, Peer M. (2014) A comparative study: The impact of different lipid extraction methods on current microalgal lipid research. Microbial Cell Factories, 13 1: . doi:10.1186/1475-2859-13-14

Author Li, Yan
Ghasemi Naghdi, Forough
Garg, Sourabh
Adarme-Vega, Tania Catalina
Thurecht, Kristofer J.
Ghafor, Wael Abdul
Tannock, Simon
Schenk, Peer M.
Title A comparative study: The impact of different lipid extraction methods on current microalgal lipid research
Journal name Microbial Cell Factories   Check publisher's open access policy
ISSN 1475-2859
Publication date 2014-01-24
Year available 2014
Sub-type Article (original research)
DOI 10.1186/1475-2859-13-14
Open Access Status DOI
Volume 13
Issue 1
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central Ltd.
Language eng
Subject 1305 Biotechnology
1502 Banking, Finance and Investment
2402 Applied Microbiology and Biotechnology
Abstract Microalgae cells have the potential to rapidly accumulate lipids, such as triacylglycerides that contain fatty acids important for high value fatty acids (e.g., EPA and DHA) and/or biodiesel production. However, lipid extraction methods for microalgae cells are not well established, and there is currently no standard extraction method for the determination of the fatty acid content of microalgae. This has caused a few problems in microlagal biofuel research due to the bias derived from different extraction methods. Therefore, this study used several extraction methods for fatty acid analysis on marine microalga Tetraselmis sp. M8, aiming to assess the potential impact of different extractions on current microalgal lipid research. These methods included classical Bligh & Dyer lipid extraction, two other chemical extractions using different solvents and sonication, direct saponification and supercritical CO2 extraction. Soxhlet-based extraction was used to weigh out the importance of solvent polarity in the algal oil extraction. Coupled with GC/MS, a Thermogravimetric Analyser was used to improve the quantification of microalgal lipid extractions. Among these extractions, significant differences were observed in both, extract yield and fatty acid composition. The supercritical extraction technique stood out most for effective extraction of microalgal lipids, especially for long chain unsaturated fatty acids. The results highlight the necessity for comparative analyses of microalgae fatty acids and careful choice and validation of analytical methodology in microalgal lipid research.
Keyword Extract yield
Fatty acid
Lipid profile
Microalgal oil
Solvent polarity
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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