Surgical models for cardiac regeneration in neonatal mice

Mahmoud, Ahmed I., Porrello, Enzo R., Kimura, Wataru, Olson, Eric N. and Sadek, Hesham A. (2014) Surgical models for cardiac regeneration in neonatal mice. Nature Protocols, 9 2: 305-311. doi:10.1038/nprot.2014.021

Author Mahmoud, Ahmed I.
Porrello, Enzo R.
Kimura, Wataru
Olson, Eric N.
Sadek, Hesham A.
Title Surgical models for cardiac regeneration in neonatal mice
Journal name Nature Protocols   Check publisher's open access policy
ISSN 1754-2189
Publication date 2014-02-01
Year available 2014
Sub-type Article (original research)
DOI 10.1038/nprot.2014.021
Open Access Status Not yet assessed
Volume 9
Issue 2
Start page 305
End page 311
Total pages 7
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 1300 Biochemistry, Genetics and Molecular Biology
Abstract Although amphibian and fish models of heart regeneration have existed for decades, a mammalian equivalent has long remained elusive. Our discovery of a brief postnatal window for heart regeneration in neonatal mice has led to the establishment of surgical models for cardiac regenerative studies in mammals for the first time. This protocol describes a 10-min surgical procedure to induce cardiac injury in 1-d-old neonatal mice. This allows for the analysis of cardiac regeneration after surgical amputation of the left ventricle (LV) (apical resection) and coronary artery occlusion (myocardial infarction (MI)). A comparative analysis of neonatal and adult responses to myocardial injury should enable identification of the key differences between regenerative and nonregenerative responses to cardiac injury. This protocol can also be adapted to the growing repertoire of genetic models available in the mouse, and it provides a valuable tool for unlocking the molecular mechanisms that guide mammalian heart regeneration during early postnatal life.
Keyword Cardiac regeneration
Neonatal mice
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Biomedical Sciences Publications
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