Low temperature alters plasma membrane lipid composition and ATPase activity of pineapple fruit during blackheart development

Zhou, Yuchan, Pan, Xiaoping, Qu, Hongxia and Underhill, Steven J. R. (2014) Low temperature alters plasma membrane lipid composition and ATPase activity of pineapple fruit during blackheart development. Journal of Bioenergetics and Biomembranes, 46 1: 59-69. doi:10.1007/s10863-013-9538-4


Author Zhou, Yuchan
Pan, Xiaoping
Qu, Hongxia
Underhill, Steven J. R.
Title Low temperature alters plasma membrane lipid composition and ATPase activity of pineapple fruit during blackheart development
Journal name Journal of Bioenergetics and Biomembranes   Check publisher's open access policy
ISSN 0145-479X
1573-6881
Publication date 2014-01-01
Year available 2014
Sub-type Article (original research)
DOI 10.1007/s10863-013-9538-4
Open Access Status DOI
Volume 46
Issue 1
Start page 59
End page 69
Total pages 11
Place of publication New York, NY United States
Publisher Springer New York LLC
Language eng
Subject 1314 Physiology
1307 Cell Biology
Formatted abstract
Plasma membrane (PM) plays central role in triggering primary responses to chilling injury and sustaining cellular homeostasis. Characterising response of membrane lipids to low temperature can provide important information for identifying early causal factors contributing to chilling injury. To this end, PM lipid composition and ATPase activity were assessed in pineapple fruit (Ananas comosus) in relation to the effect of low temperature on the development of blackheart, a form of chilling injury. Chilling temperature at 10 C induced blackheart development in concurrence with increase in electrolyte leakage. PM ATPase activity was decreased after 1 week at low temperature, followed by a further decrease after 2 weeks. The enzyme activity was not changed during 25 °C storage. Loss of total PM phospholipids was found during postharvest senescence, but more reduction was shown from storage at 10 °C. Phosphatidylcholine and phosphatidylethanolamine were the predominant PM phospholipid species. Low temperature increased the level of phosphatidic acid but decreased the level of phosphatidylinositol. Both phospholipid species were not changed during storage at 25 °C. Postharvest storage at both temperatures decreased the levels of C18:3 and C16:1, and increased level of C18:1. Low temperature decreased the level of C18:2 and increased the level of C14:0. Exogenous application of phosphatidic acid was found to inhibit the PM ATPase activity of pineapple fruit in vitro. Modification of membrane lipid composition and its effect on the functional property of plasma membrane at low temperature were discussed in correlation with their roles in blackheart development of pineapple fruit.
Keyword Plasma membrane
ATPase
Lipid composition
Blackheart (chilling injury)
Pineapple fruit
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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