Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability

Meador, Travis B., Gagen, Emma J., Loscar, Michael E., Goldhammer, Tobias, Yoshinaga, Marcos Y., Wendt, Jenny, Thomm, Michael and Hinrichs, Kai-Uwe (2014) Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability. Frontiers in Microbiology, 5 . doi:10.3389/fmicb.2014.00010


Author Meador, Travis B.
Gagen, Emma J.
Loscar, Michael E.
Goldhammer, Tobias
Yoshinaga, Marcos Y.
Wendt, Jenny
Thomm, Michael
Hinrichs, Kai-Uwe
Title Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability
Formatted title
Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability
Journal name Frontiers in Microbiology   Check publisher's open access policy
ISSN 1664-302X
Publication date 2014-01-30
Year available 2014
Sub-type Article (original research)
DOI 10.3389/fmicb.2014.00010
Open Access Status DOI
Volume 5
Total pages 14
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Language eng
Formatted abstract
We observed significant changes in the elemental and intact polar lipid (IPL) composition of the archaeon Thermococcus kodakarensis (KOD1) in response to growth stage and phosphorus supply. Reducing the amount of organic supplements and phosphate in growth media resulted in significant decreases in cell size and cellular quotas of carbon (C), nitrogen (N), and phosphorus (P), which coincided with significant increases in cellular IPL quota and IPLs comprising multiple P atoms and hexose moieties. Relatively more cellular P was stored as IPLs in P-limited cells (2-8%) compared to control cells (<0.8%). We also identified a specific IPL biomarker containing a phosphatidyl-N-acetylhexoseamine headgroup that was relatively enriched during rapid cell division. These observations serve as empirical evidence of IPL adaptations in Archaea that will help to interpret the distribution of these biomarkers in natural systems. The reported cell quotas of C, N, and P represent the first such data for a specific archaeon and suggest that thermophiles are C-rich compared to the cell carbon-to-volume relationship reported for planktonic bacteria. 
Keyword Biomarker
Cell carbon quota
Phosphorus limitation
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 247153
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Earth Sciences Publications
Non HERDC
 
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