Aberrant gonadotropin-releasing hormone receptor (GnRHR) expression and its regulation of CYP11B2 expression and aldosterone production in adrenal aldosterone-producing adenoma (APA)

Nakamura, Yasuhiro, Hattangady, Namita G., Ye, Ping, Satoh, Fumitoshi, Morimoto, Ryo, Ito-Saito, Takako, Sugawara, Akira, Ohba, Koji, Takahashi, Kazuhiro, Rainey, William E. and Sasano H. (2014) Aberrant gonadotropin-releasing hormone receptor (GnRHR) expression and its regulation of CYP11B2 expression and aldosterone production in adrenal aldosterone-producing adenoma (APA). Molecular and Cellular Endocrinology, 384 1-2: 102-108. doi:10.1016/j.mce.2014.01.016


Author Nakamura, Yasuhiro
Hattangady, Namita G.
Ye, Ping
Satoh, Fumitoshi
Morimoto, Ryo
Ito-Saito, Takako
Sugawara, Akira
Ohba, Koji
Takahashi, Kazuhiro
Rainey, William E.
Sasano H.
Title Aberrant gonadotropin-releasing hormone receptor (GnRHR) expression and its regulation of CYP11B2 expression and aldosterone production in adrenal aldosterone-producing adenoma (APA)
Journal name Molecular and Cellular Endocrinology   Check publisher's open access policy
ISSN 0303-7207
1872-8057
Publication date 2014-03-25
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.mce.2014.01.016
Volume 384
Issue 1-2
Start page 102
End page 108
Total pages 7
Place of publication Shannon, Co. Clare, Ireland
Publisher Elsevier
Collection year 2015
Language eng
Formatted abstract
Aberrant expression of gonadotropin-releasing hormone receptor (GnRHR) has been reported in human adrenal tissues including aldosterone-producing adenoma (APA). However, the details of its expression and functional role in adrenals are still not clear. In this study, quantitative RT-PCR analysis revealed the mean level of GnRHR mRNA was significantly higher in APAs than in human normal adrenal (NA) (P=0.004). GnRHR protein expression was detected in human NA and neoplastic adrenal tissues. In H295R cells transfected with GnRHR, treatment with GnRH resulted in a concentration-dependent increase in CYP11B2 reporter activity. Chronic activation of GnRHR with GnRH (100. nM), in a cell line with doxycycline-inducible GnRHR (H295R-TR/GnRHR), increased CYP11B2 expression and aldosterone production. These agonistic effects were inhibited by blockers for the calcium signaling pathway, KN93 and calmidazolium. These results suggest GnRH, through heterotopic expression of its receptor, may be a potential regulator of CYP11B2 expression levels in some cases of APA.
Keyword Adrenal aldosterone-producing adenoma (APA)
Aldosterone
CYP11B2
Gonadotropin-releasing hormone receptor (GnRHR)
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Pharmacy Publications
Official 2015 Collection
 
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