Opportunistic pathogens in roof-captured rainwater samples, determined using quantitative PCR

Ahmed ,W., Brandes, H., Gyawali, P., Sidhu, J. P. S. and Toze, S. (2014) Opportunistic pathogens in roof-captured rainwater samples, determined using quantitative PCR. Water Research, 53 361-369. doi:10.1016/j.watres.2013.12.021


Author Ahmed ,W.
Brandes, H.
Gyawali, P.
Sidhu, J. P. S.
Toze, S.
Title Opportunistic pathogens in roof-captured rainwater samples, determined using quantitative PCR
Journal name Water Research   Check publisher's open access policy
ISSN 0043-1354
1879-2448
Publication date 2014-04-15
Year available 2014
Sub-type Article (original research)
DOI 10.1016/j.watres.2013.12.021
Open Access Status Not Open Access
Volume 53
Start page 361
End page 369
Total pages 9
Place of publication London, United Kingdom
Publisher I W A Publishing
Language eng
Subject 2312 Water Science and Technology
2311 Waste Management and Disposal
2310 Pollution
2302 Ecological Modelling
Abstract In this study, quantitative PCR (qPCR) was used for the detection of four opportunistic bacterial pathogens in water samples collected from 72 rainwater tanks in Southeast Queensland, Australia. Tank water samples were also tested for fecal indicator bacteria (Escherichia coli and Enterococcus spp.) using culture-based methods. Among the 72 tank water samples tested, 74% and 94% samples contained E. coli and Enterococcus spp., respectively, and the numbers of E. coli and Enterococcus spp. in tank water samples ranged from 0.3 to 3.7 log10 colony forming units (CFU) per 100mL of water. In all, 29%, 15%, 13%, and 6% of tank water samples contained Aeromonas hydrophila, Staphylococcus aureus, Pseudomonas aeruginosa and Legionella pneumophila, respectively. The genomic units (GU) of opportunistic pathogens in tank water samples ranged from 1.5 to 4.6 log10 GU per 100mL of water. A significant correlation was found between E. coli and Enterococcus spp. numbers in pooled tank water samples data (Spearman's rs=0.50; P<0.001). In contrast, fecal indicator bacteria numbers did not correlate with the presence/absence of opportunistic pathogens tested in this study. Based on the results of this study, it would be prudent, to undertake a Quantitative Microbial Risk Assessment (QMRA) analysis of opportunistic pathogens to determine associated health risks for potable and nonpotable uses of tank water.
Keyword Fecal indicator bacteria
Health risks
Opportunistic pathogens
Quantitative PCR
Roof-captured rainwater
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Public Health Publications
 
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