Reduced secretagogin expression in the hippocampus of P301L tau transgenic mice

Attems, Johannes, Ittner, Arne, Jellinger, Kurt, Nitsch, Roger M., Maj, Magdalena, Wagner, Ludwig, Gotz, Jurgen and Heikenwalder, Mathias (2011) Reduced secretagogin expression in the hippocampus of P301L tau transgenic mice. Journal of Neural Transmission, 118 5: 737-745. doi:10.1007/s00702-011-0626-5


Author Attems, Johannes
Ittner, Arne
Jellinger, Kurt
Nitsch, Roger M.
Maj, Magdalena
Wagner, Ludwig
Gotz, Jurgen
Heikenwalder, Mathias
Title Reduced secretagogin expression in the hippocampus of P301L tau transgenic mice
Journal name Journal of Neural Transmission   Check publisher's open access policy
ISSN 0300-9564
1435-1463
Publication date 2011-01-01
Sub-type Article (original research)
DOI 10.1007/s00702-011-0626-5
Volume 118
Issue 5
Start page 737
End page 745
Total pages 9
Place of publication Wien, Austria
Publisher Springer
Language eng
Abstract Neuropathological features in Alzheimer's Disease (AD) include the presence of hyperphosphorylated forms of the microtubule-associated tau protein (tau) in hippocampal neurones. Numerous studies indicate a neuroprotective effect of calcium-binding proteins (Ca2+ binding proteins) in neurodegenerative diseases (e.g., AD). Secretagogin is a newly described Ca2+ binding protein that is produced by pyramidal neurones of the human hippocampus. Recently, secretagogin expressing hippocampal neurones were demonstrated to resist tau-induced pathology in AD in contrast to the majority of neighbouring neurones. This suggested a neuroprotective effect of secretagogin in hippocampal neurones. Here, we investigated secretagogin expression in wild type (wt) mice as well as in hemizygous and homozygous P301L tau transgenic (tg) mice, which show pronounced and widespread tau pathology in hippocampal neurones. Secretagogin expression was analyzed at the immunohistochemical and biochemical levels in brains of age-matched wt and hemi- and homozygous tau tg mice. In wt mice hippocampal secretagogin-immunoreactive neurones were invariably detected, while immunoreactivity was much lower (P < 0.001) in tau tg mice. Of note, hippocampal secretagogin immunoreactivity was absent in 62.5% of homozygous tau tg mice. In line with this finding, Western blot analysis demonstrated a significant reduction in protein expression levels of secretagogin in homozygous tau tg compared to wt mice. Our results suggest that increased levels of tau negatively influence secretagogin expression in the hippocampus of tau tg mice.
Keyword Alzheimer's disease
Calcium-binding protein
P301L tau transgenic mouse
Secretagogin
Tau pathology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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