Genomic organisation, chromosomal localisation tissue distribution and developmental regulation of the PR61/B′ regulatory subunits of protein phosphatase 2A in mice

Martens, Ellen, Stevens, Ilse, Janssens, Veerle, Vermeesch, Joris, Gotz, Juergen, Goris, Jozef and Van Hoof , Christine Van (2004) Genomic organisation, chromosomal localisation tissue distribution and developmental regulation of the PR61/B′ regulatory subunits of protein phosphatase 2A in mice. Journal of Molecular Biology, 336 4: 971-986. doi:10.1016/j.jmb.2003.12.047


Author Martens, Ellen
Stevens, Ilse
Janssens, Veerle
Vermeesch, Joris
Gotz, Juergen
Goris, Jozef
Van Hoof , Christine Van
Title Genomic organisation, chromosomal localisation tissue distribution and developmental regulation of the PR61/B′ regulatory subunits of protein phosphatase 2A in mice
Journal name Journal of Molecular Biology   Check publisher's open access policy
ISSN 0022-2836
1089-8638
Publication date 2004-02-27
Year available 2004
Sub-type Article (original research)
DOI 10.1016/j.jmb.2003.12.047
Open Access Status Not Open Access
Volume 336
Issue 4
Start page 971
End page 986
Total pages 16
Place of publication London, United Kingdom
Publisher Academic Press
Language eng
Abstract Protein phosphatase 2A (PP2A) is a major serine/threonine-specific phosphatase playing central roles in development, cell growth and transformation. Regulation is largely accomplished by the regulatory B subunits, which determine substrate specificity, subcellular localisation and catalytic activity. The B′ family, also known as the PR61 family, is the most diverse, consisting of five genes (α,β,γ,δ and ε) that give rise to a number of splice variants. We deduced the sequences of the different PR61 proteins in mice and found evidence for the expression of PR61α, β1, γ1, γ2, γ3, δ1 and ε. We report the genomic organisation and localisation of the murine PR61 genes (Ppp2r5a-Ppp2r5e). This information will be useful for the future realisation of PR61 knockouts. Using Northern blotting, we examined the expression of the five PR61 isoforms in different tissues. A brain-specific function can be expected for the PR61β protein based on the high expression levels observed in murine brain. In situ hybridisation analysis of the adult brain revealed a distinct and partially overlapping pattern of mRNA expression of the various PR61 isoforms. The PR61 mRNA expression during embryonic development was examined by Northern blotting. The PR61 transcripts were differentially expressed, suggesting a specific function for each of the PR61 proteins during embryonic development and/or adult life.
Keyword PP2A
PR61/B′
Genomic organisation
Expression patterns
Developmental regulation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Queensland Brain Institute Publications
 
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