Pronuclear injection for the production of transgenic mice

Ittner, Lars M. and Gotz, Juergen (2007) Pronuclear injection for the production of transgenic mice. Nature Protocols, 2 5: 1206-1215. doi:10.1038/nprot.2007.145


Author Ittner, Lars M.
Gotz, Juergen
Title Pronuclear injection for the production of transgenic mice
Journal name Nature Protocols   Check publisher's open access policy
ISSN 1754-2189
1750-2799
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1038/nprot.2007.145
Open Access Status Not yet assessed
Volume 2
Issue 5
Start page 1206
End page 1215
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Abstract Transgenic mice have been instrumental in dissecting the role of various neuronal proteins under both physiological and pathological conditions. Pronuclear injection is the most widely used protocol for the generation of transgenic mice. Here, we describe all steps involved from DNA purification to the set up of a mouse colony including vasectomy, injection of the DNA into a donor zygote, transfer of injected zygotes into recipient foster mice, screening of offspring and establishment of transgenic mouse lines. We discuss the use of neuron-specific promoters to express proteins with a role in Alzheimer disease. Transgenic expression of a truncated form of the microtubule-associated protein tau (Δtau) is used as an example for the anticipated results.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Ageing Dementia Research Publications
 
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