An evaluation of serotyping of Avibacterium paragallinarum by use of a multiplex polymerase chain reaction

Morales-Erasto, Vladimir, Posadas-Quintana, José de Jesús, Fernández-Díaz, Manolo, Saravia, Luis E., Martínez-Castañeda, José Simón, Blackall, Patrick J. and Soriano-Vargas, Edgardo (2014) An evaluation of serotyping of Avibacterium paragallinarum by use of a multiplex polymerase chain reaction. Journal of Veterinary Diagnostic Investigation, OnlineFirst 2: 1-6. doi:10.1177/1040638714523612

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Author Morales-Erasto, Vladimir
Posadas-Quintana, José de Jesús
Fernández-Díaz, Manolo
Saravia, Luis E.
Martínez-Castañeda, José Simón
Blackall, Patrick J.
Soriano-Vargas, Edgardo
Title An evaluation of serotyping of Avibacterium paragallinarum by use of a multiplex polymerase chain reaction
Formatted title
An evaluation of serotyping of Avibacterium paragallinarum by use of a multiplex polymerase chain reaction
Journal name Journal of Veterinary Diagnostic Investigation   Check publisher's open access policy
ISSN 1040-6387
1943-4936
Publication date 2014-03-03
Sub-type Article (original research)
DOI 10.1177/1040638714523612
Volume OnlineFirst
Issue 2
Start page 1
End page 6
Total pages 6
Place of publication Thousand Oaks, CA, United States
Publisher Sage Publications
Language eng
Formatted abstract
In the present study, the ability of a recently proposed multiplex polymerase chain reaction (mPCR) to determine the serogroups (A, B, and C) of Avibacterium paragallinarum was evaluated. A total of 12 reference strains and 69 field isolates of Av. paragallinarum from Ecuador, Mexico, Panama, and Peru were included in the study. With some exceptions (which were serotyped in the current study), all of the isolates and strains had been previously examined by 2 serotyping schemes (Page and Kume) or were the formal reference strains for the schemes. Three of 6 (50%) reference strains of serogroup A, 2 (100%) of serogroup B, and 1 of 4 (25%) reference strains of serogroup C were correctly serotyped by the mPCR. With the field isolates, the mPCR correctly recognized 16 of the 17 serogroup A isolates, 10 of the 12 serogroup B isolates, and 18 of the 37 serogroup C isolates. Overall, the specificity and sensitivity of the PCR test was as follows: 82.6% and 87.3% (serogroup A), 85.7% and 71.9% (serogroup B), and 46.3% and 100% (serogroup C). The poor performance of the mPCR in terms of recognition of serogroup C isolates (low sensitivity of 46.3%) and the relatively high level of uncertainty about the accuracy of the serogroup A and B results (specificity of 87.3% and 71.9%, respectively) means that the assay cannot be recommended as a replacement for conventional serotyping.
Keyword Avibacterium paragallinarum
Infectious coryza
Polymerase chain reaction
Poultry
Serotyping
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online before print March 3, 2014

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
School of Veterinary Science Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
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Created: Wed, 19 Feb 2014, 01:17:22 EST by Dr Patrick Blackall on behalf of Qld Alliance for Agriculture and Food Innovation