Use of REP-PCR and 16S rRNA gene sequencing for comparison of Mannheimia haemolytica isolates obtained from fatal cases of bovine respiratory disease in the USA and Australia

Taylor, J. D., Doyle, D. J., Blackall, P. J. and Confer A. (2014) Use of REP-PCR and 16S rRNA gene sequencing for comparison of Mannheimia haemolytica isolates obtained from fatal cases of bovine respiratory disease in the USA and Australia. Australian Veterinary Journal, 92 1-2: 15-23. doi:10.1111/avj.12137


Author Taylor, J. D.
Doyle, D. J.
Blackall, P. J.
Confer A.
Title Use of REP-PCR and 16S rRNA gene sequencing for comparison of Mannheimia haemolytica isolates obtained from fatal cases of bovine respiratory disease in the USA and Australia
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
1751-0813
Publication date 2014-01-01
Sub-type Article (original research)
DOI 10.1111/avj.12137
Volume 92
Issue 1-2
Start page 15
End page 23
Total pages 9
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell
Language eng
Subject 3400 Veterinary
Formatted abstract
Objective: Assess the variability of Mannheimia haemolytica isolates obtained from fatal cases of bovine respiratory disease (BRD) in the USA and Australia using repetitive sequence-based PCR (REP-PCR) and sequencing of the 16S ribosomal RNA (rRNA) gene.

Methods: We examined 22 isolates from the USA and 36 isolates from Australia using (GTG)5 and BOX-A1R REP-PCR primers, as well as sequencing a 700-base pair length of the 16S rRNA gene. The discriminatory ability of each typing method was assessed and correlation coefficients were calculated to assess concordance between the results of each approach.

Results: All methods appeared to discriminate among isolates, with BOX-A1R being the most sensitive and sequencing the least sensitive. Modest to moderate diversity was seen among the isolates, with as much variation within a continent as between the two.

Conclusions: Using samples from diverse origins may permit extrapolation even to isolates with distant geographic and temporal relationships. Further, this information can serve as a baseline in assessing whether M. haemolytica is an opportunistic pathogen or if there are notable features that distinguish commensal isolates from those more likely to be associated with disease.
Keyword Bovine respiratory disease
Mannheimia haemolytica
Molecular epidemiology
REP-PCR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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