Morphological and cellular changes in secondary epidermal laminae of horses with insulin-induced laminitis

Karikoski, Ninja P., Patterson-Kane, Janet C., Asplin, Katie E, McGowan, Thomas W., McNutt, Megan, Singer, Ellen R. and McGowan, Catherine M. (2014) Morphological and cellular changes in secondary epidermal laminae of horses with insulin-induced laminitis. American Journal of Veterinary Research, 75 2: 161-168. doi:10.2460/ajvr.75.2.161


Author Karikoski, Ninja P.
Patterson-Kane, Janet C.
Asplin, Katie E
McGowan, Thomas W.
McNutt, Megan
Singer, Ellen R.
McGowan, Catherine M.
Title Morphological and cellular changes in secondary epidermal laminae of horses with insulin-induced laminitis
Journal name American Journal of Veterinary Research   Check publisher's open access policy
ISSN 0002-9645
1943-5681
Publication date 2014-02-01
Year available 2014
Sub-type Article (original research)
DOI 10.2460/ajvr.75.2.161
Volume 75
Issue 2
Start page 161
End page 168
Total pages 8
Place of publication Schaumburg, United States
Publisher American Veterinary Medical Association
Collection year 2014
Language eng
Formatted abstract
Objective—To determine cellular changes associated with secondary epidermal laminae (SEL) in forefeet and hind feet of ponies with insulin-induced laminitis.

Animals—8 ponies.

Procedures—Laminitis was induced in 4 ponies by IV administration of insulin and glucose; 4 control ponies received saline (0.9% NaCl) solution IV. Laminar tissue samples obtained from the dorsal aspects of the hooves were histologically evaluated. Primary epidermal lamina (PEL) length and width and SEL length, width, and angle were determined. Numbers of epidermal cell nuclei per micrometer and per total length of SEL and numbers of apoptotic and proliferative cells in axial, middle, and abaxial laminar regions were determined.

Results—SEL in treatment group ponies were significantly longer, were significantly narrower, and had a smaller angle relative to PEL in all laminar regions versus control ponies. In treatment group ponies, the number of epidermal cell nuclei per SEL was typically higher and the number of cells per micrometer of SEL was lower in laminar regions, apoptotic cell numbers were higher in abaxial and middle regions in forefeet and hind feet, and proliferating cell numbers were higher in axial laminar regions in forefeet and all laminar regions in hind feet, versus control ponies.

Conclusions and Clinical Relevance—Results indicated SEL elongation, narrowing, and alteration in orientation developed in all feet of ponies with insulin-induced laminitis. This was primarily attributable to cell stretching that developed at the same time as an accelerated cell death–proliferation cycle; differences in cell cycle responses among laminar regions between forefeet and hind feet may have been attributable to differences in load bearing.

Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2015 Collection
School of Veterinary Science Publications
 
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