GABAergic agents modify the response of chick scleral fibroblasts to myopic and hyperopic eye cup tissues

Christian, Parul G., Harkin, Damien G. and Schmid, Katrina L. (2014) GABAergic agents modify the response of chick scleral fibroblasts to myopic and hyperopic eye cup tissues. Current Eye Research, 39 2: 172-187. doi:10.3109/02713683.2013.834941

Author Christian, Parul G.
Harkin, Damien G.
Schmid, Katrina L.
Title GABAergic agents modify the response of chick scleral fibroblasts to myopic and hyperopic eye cup tissues
Journal name Current Eye Research   Check publisher's open access policy
ISSN 0271-3683
Publication date 2014-02-01
Year available 2013
Sub-type Article (original research)
DOI 10.3109/02713683.2013.834941
Open Access Status
Volume 39
Issue 2
Start page 172
End page 187
Total pages 16
Place of publication London, UK
Publisher Informa Healthcare
Language eng
Formatted abstract
Purpose: GABA antagonists inhibit experimental myopia in chick and GABA receptors have been localized to chick sclera and the retinal pigment epithelium (RPE). The RPE and the choroid alter scleral DNA and glycosaminoglycan (GAG) content in vitro; opposite effects have been observed for tissues from myopic and hyperopic eyes. The aim was to determine the effect of GABAergic agents on the DNA and GAG content of chick scleral fibroblasts directly and in co-culture with ocular tissues from myopic and hyperopic chick eyes.

Materials and Methods: Primary cultures of fibroblastic cells expressing vimentin and α-smooth muscle actin were established. GABAergic agents were added separately (i) to the culture medium of the scleral cells and (ii) to the culture medium of the scleral cells with the addition of posterior eye cup tissue (retina, RPE, retina+RPE, choroid+RPE) to cell culture inserts. Ocular tissues were obtained from chick eyes wearing+15D (lens-induced hyperopia, LIH) or -15D lenses (lens-induced myopia, LIM) for three days (post-hatch day 5-8) (n=12). GAG and DNA content of scleral fibroblasts were measured.

Results: GABA agents had a small direct effect on scleral cell GAG and DNA content but a larger effect was measured when GABA agents were added to the culture medium with myopic and hyperopic RPE and choroid+RPE tissues. GABA agonists increased (p=0.002) whereas antagonists decreased (p=0.0004) DNA content of scleral cells; effects were opposite for scleral GAG content. GABA agents significantly altered the effect of both LIM and LIH tissues (p=0.0005) compared to control; the effects were greater for LIM tissue versus LIH tissue co-culture (p=0.0004).

Conclusion: GABAergic agents affect the DNA and GAG content of scleral fibroblasts both directly and when co-cultured with ocular tissues. GABA antagonists that prevent myopia development in chick model could act via a scleral mechanism utilizing the RPE/choroid.
Keyword Chick
Gamma-amino butyric acid
Refractive error
Retinal pigment epithelium
Scleral fibroblasts
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Medicine Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 18 Feb 2014, 10:22:26 EST by System User on behalf of Scholarly Communication and Digitisation Service