Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades

Jesupret, Clémence, Baumann, Kate, Jackson, Timothy N. W., Ali, Syed Abid, Yang, Daryl C., Greisman, Laura, Kern, Larissa, Steuten, Jessica, Jouiaei, Mahdokht, Casewell, Nicholas R., Undheim, Eivind A. B., Koludarov, Ivan, Debono, Jordan, Low, Dolyce H. W., Rossi, Sarah, Panagides, Nadya, Winter, Kelly, Ignjatovic, Vera, Summerhayes, Robyn, Jones, Alun, Nouwens, Amanda, Dunstan, Nathan, Hodgson, Wayne C., Winkel, Kenneth D., Monagle, Paul and Fry, Bryan Grieg (2014) Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades. Journal of Proteomics, 105 285-294. doi:10.1016/j.jprot.2014.01.004

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Author Jesupret, Clémence
Baumann, Kate
Jackson, Timothy N. W.
Ali, Syed Abid
Yang, Daryl C.
Greisman, Laura
Kern, Larissa
Steuten, Jessica
Jouiaei, Mahdokht
Casewell, Nicholas R.
Undheim, Eivind A. B.
Koludarov, Ivan
Debono, Jordan
Low, Dolyce H. W.
Rossi, Sarah
Panagides, Nadya
Winter, Kelly
Ignjatovic, Vera
Summerhayes, Robyn
Jones, Alun
Nouwens, Amanda
Dunstan, Nathan
Hodgson, Wayne C.
Winkel, Kenneth D.
Monagle, Paul
Fry, Bryan Grieg
Title Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades
Journal name Journal of Proteomics   Check publisher's open access policy
ISSN 1874-3919
1876-7737
Publication date 2014-01-14
Sub-type Article (original research)
DOI 10.1016/j.jprot.2014.01.004
Open Access Status File (Author Post-print)
Volume 105
Start page 285
End page 294
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
For over a century, venom samples from wild snakes have been collected and stored around the world. However, the quality of storage conditions for “vintage” venoms has rarely been assessed. The goal of this study was to determine whether such historical venom samples are still biochemically and pharmacologically viable for research purposes, or if new sample efforts are needed. In total, 52 samples spanning 5 genera and 13 species with regional variants of some species (e.g., 14 different populations of Notechis scutatus) were analysed by a combined proteomic and pharmacological approach to determine protein structural stability and bioactivity. When venoms were not exposed to air during storage, the proteomic results were virtually indistinguishable from that of fresh venom and bioactivity was equivalent or only slightly reduced. By contrast, a sample of Acanthophis antarcticus venom that was exposed to air (due to a loss of integrity of the rubber stopper) suffered significant degradation as evidenced by the proteomics profile. Interestingly, the neurotoxicity of this sample was nearly the same as fresh venom, indicating that degradation may have occurred in the free N- or C-terminus chains of the proteins, rather than at the tips of loops where the functional residues are located. These results suggest that these and other vintage venom collections may be of continuing value in toxin research. This is particularly important as many snake species worldwide are declining due to habitat destruction or modification. For some venoms (such as N. scutatus from Babel Island, Flinders Island, King Island and St. Francis Island) these were the first analyses ever conducted and these vintage samples may represent the only venom ever collected from these unique island forms of tiger snakes. Such vintage venoms may therefore represent the last remaining stocks of some local populations and thus are precious resources. These venoms also have significant historical value as the Oxyuranus venoms analysed include samples from the first coastal taipan (Oxyuranus scutellatus) collected for antivenom production (the snake that killed the collector Kevin Budden), as well as samples from the first Oxyuranus microlepidotus specimen collected after the species' rediscovery in 1976. These results demonstrate that with proper storage techniques, venom samples can retain structural and pharmacological stability. 
Keyword Venom
Protein
1D gel
Mass spectrometry
Toxinology
Stability studies
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes This article is part of a Special Issue entitled: proteomics of non-model organisms

 
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Created: Fri, 14 Feb 2014, 20:33:06 EST by Mrs Louise Nimwegen on behalf of School of Biological Sciences