Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans

Watamoto, T., Samaranayake, L. P., Jayatilake, J. A. M. S., Egusa, H., Yatani, H. and Seneviratne, C. J. (2009) Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans. International Journal of Antimicrobial Agents, 34 4: 333-339. doi:10.1016/j.ijantimicag.2009.03.008


Author Watamoto, T.
Samaranayake, L. P.
Jayatilake, J. A. M. S.
Egusa, H.
Yatani, H.
Seneviratne, C. J.
Title Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans
Formatted title
Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans 
Journal name International Journal of Antimicrobial Agents   Check publisher's open access policy
ISSN 0924-8579
1872-7913
Publication date 2009-01-01
Sub-type Article (original research)
DOI 10.1016/j.ijantimicag.2009.03.008
Volume 34
Issue 4
Start page 333
End page 339
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Biofilm formation involving profuse hyphal growth is a major characteristic of Candida spp. and confers higher antifungal resistance than its planktonic mode of growth. We investigated the antifungal susceptibility of Candida albicans and its hyphal mutants (Δefg1/efg1, Δcph1/cph1 and ΔΔcph1/cph1 efg1/efg1) to commonly used antifungals during planktonic, adhesion and biofilm modes of growth. The minimum inhibitory concentration (MIC) of each antifungal agent was determined for a lower inoculum (1 × 10 3 cells/mL) and higher inoculum (1 × 10 7 cells/mL) of planktonic Candida. Furthermore, MICs of C. albicans biofilms and adhesion modes of growth were determined with a standard XTT assay. Candida albicans in adhesion and biofilm modes of growth, but not in planktonic mode, were resistant to all five antifungal agents tested. Although Δefg1/efg1 and ΔΔcph1/cph1 efg1/efg1 mutants formed less biofilm than wild-type C. albicans SC5314, they were similarly resistant to caspofungin. However, these mutants were more sensitive to amphotericin B and nystatin than the wild-type. Adhesion per se confers increased resistance to antifungal agents, which is further pronounced in the biofilm mode of Candida. Filamentation does not appear to be a major determinant of the antifungal resistance in Candida biofilms.
Keyword Antifungal susceptibility
Biofilm
Candida
Hyphal mutants
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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