Proposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases

Taniguchi, Lisa, de Fatima Faria, Berenice, Rosa, Rosimeire Takaki, de Paula e Carvalho, Alessandra, Gursky, Lauren Christine, Elifio-Esposito, Selene Lobo, Parahitiyawa, Nipuna, Samaranayake, Lakshman Perera and Ribeiro Rosa, Edvaldo Antonio (2009) Proposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases. Journal of Microbiological Methods, 78 2: 171-174. doi:10.1016/j.mimet.2009.05.012


Author Taniguchi, Lisa
de Fatima Faria, Berenice
Rosa, Rosimeire Takaki
de Paula e Carvalho, Alessandra
Gursky, Lauren Christine
Elifio-Esposito, Selene Lobo
Parahitiyawa, Nipuna
Samaranayake, Lakshman Perera
Ribeiro Rosa, Edvaldo Antonio
Title Proposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases
Formatted title
Proposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases
Journal name Journal of Microbiological Methods   Check publisher's open access policy
ISSN 0167-7012
1872-8359
Publication date 2009-01-01
Sub-type Article (original research)
DOI 10.1016/j.mimet.2009.05.012
Volume 78
Issue 2
Start page 171
End page 174
Total pages 4
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Biofilms are aggregates of microorganisms living in multilayered structures inside polymeric matrices onto surfaces. These biofilms may subvert the physiological properties of adjacent tissues causing morphofunctional failure. Many studies have shown that the expression of virulence attributes is maximized when microbes form such communities. This study evaluated the differential phospholipasic activity of Candida albicans SC5314 grown in planktonic phase and in biofilm. We propose two distinct protocols for the colorimetric evaluation of phosphatidylcholine hydrolysis in neutral and acidic conditions. The results showed that both protocols are suitable for the proposed intention and that 72 h-old planktonic cultures of C. albicans SC5314 secrete higher quantities of neutral (6.42-fold) and acidic (3.85-fold) phospholipases than biofilms.
Keyword Biofilm
Candida albicans
Colorimetry
Phospholipase
Planktonic
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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