Photodynamic inactivation of Candida albicans by BAM-SiPc

So, Cheung-Wai, Tsang, Paul W. K., Lo, Pui-Chi, Seneviratne, C. J., Samaranayake, Lakshman P. and Fong, Wing-Ping (2010) Photodynamic inactivation of Candida albicans by BAM-SiPc. Mycoses, 53 3: 215-220. doi:10.1111/j.1439-0507.2008.01688.x

Author So, Cheung-Wai
Tsang, Paul W. K.
Lo, Pui-Chi
Seneviratne, C. J.
Samaranayake, Lakshman P.
Fong, Wing-Ping
Title Photodynamic inactivation of Candida albicans by BAM-SiPc
Formatted title
Photodynamic inactivation of Candida albicans by BAM-SiPc
Journal name Mycoses   Check publisher's open access policy
ISSN 0933-7407
Publication date 2010-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1111/j.1439-0507.2008.01688.x
Open Access Status
Volume 53
Issue 3
Start page 215
End page 220
Total pages 6
Place of publication Berlin, Germany
Publisher Wiley-Blackwell
Language eng
Abstract Photodynamic therapy is a treatment that combines the use of three non-toxic components, viz. photosensitiser, light and oxygen to cause localised oxidative photodamage. In the present study, the antifungal effect of the photosensitiser, BAM-SiPc, an unsymmetrical bisamino phthalocyanine, was investigated. BAM-SiPc was effective in photo-inactivating Candida albicans in a dose-dependent manner. The cell viability as determined by the clonogenic assay was reduced to c. 10% at 0.02 μmol l -1 BAM-SiPc with a total fluence of 12 J cm -2 at a cell density of 10 7 cells ml -1. A short incubation time of 5-15 min was sufficient to allow the photosensitiser to exert its optimal antifungal activity. Microscopical analysis showed that BAM-SiPc was effectively internalised by the fungal cells. Photodynamic treatment led to an increase in the intracellular reactive oxygen species level and disturbed the membrane integrity of the fungal cells.
Keyword Antifungal drug
Photodynamic therapy
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID CUHK4569/05M
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 17 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 12 Feb 2014, 19:10:11 EST by System User on behalf of School of Dentistry