In vitro growth, acidogenicity and cariogenicity of predominant human root caries flora

Shen, S., Samaranayake, L. P. and Yip, H. -K. (2004) In vitro growth, acidogenicity and cariogenicity of predominant human root caries flora. Journal of Dentistry, 32 8: 667-678. doi:10.1016/j.jdent.2004.07.002


Author Shen, S.
Samaranayake, L. P.
Yip, H. -K.
Title In vitro growth, acidogenicity and cariogenicity of predominant human root caries flora
Journal name Journal of Dentistry   Check publisher's open access policy
ISSN 0300-5712
1879-176X
Publication date 2004-11-01
Year available 2004
Sub-type Article (original research)
DOI 10.1016/j.jdent.2004.07.002
Open Access Status Not yet assessed
Volume 32
Issue 8
Start page 667
End page 678
Total pages 12
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
Streptococcus mutans (Sm), Lactobacillus acidophilus (La) and Actinomyces israelii (Ai) have been associated with root surface caries, which is an increasing problem in elderly Chinese. The aim of this study therefore, was to evaluate in vitro, the growth, acidogenicity and cariogenicity of these organisms, both in mono- and co-cultures using an in vitro model. Forty-eight root specimens were prepared using intact extracted human molars. Fresh, wild-type bacteria obtained from root caries lesions were assembled into seven experimental groups as either mono- or co-cultures and incubated with the root specimens. Appropriate controls were included. Growth curve of each experimental group was monitored for 24 h, aerobically, at 37°C using a microplate reader. The pH of the medium was recorded after 24-h incubation using a pH meter. Mean depths of artificial root lesions produced in each cultural group were measured using polarized light microscopy in specimens cut into thin sections (100±20 μm). Compared with mono-cultures, synergistic growth was observed in co-cultures of 'La+Sm', 'Ai+La' and 'Ai+La+Sm'. Mean lesion depth produced in La group was significantly shallower than other mono- or co-culture groups (p<0.01). The pH values of all culture media were similar after 24-h incubation. The current data elucidate the complex interactions of three predominant bacterial species considered prime agents of human root surface caries.
Keyword Cariogenicity and artificial caries
Microbiology
Root caries
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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