Effect of oral bacteria on growth and survival of Candida albicans biofilms

Thein, Zaw M., Samaranayake, Yuthika H. and Samaranayake, Lakshman P. (2006) Effect of oral bacteria on growth and survival of Candida albicans biofilms. Archives of Oral Biology, 51 8: 672-680. doi:10.1016/j.archoralbio.2006.02.005

Author Thein, Zaw M.
Samaranayake, Yuthika H.
Samaranayake, Lakshman P.
Title Effect of oral bacteria on growth and survival of Candida albicans biofilms
Formatted title
Effect of oral bacteria on growth and survival of Candida albicans biofilms
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
Publication date 2006-08-01
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.archoralbio.2006.02.005
Open Access Status Not yet assessed
Volume 51
Issue 8
Start page 672
End page 680
Total pages 9
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Formatted abstract
The purpose of this study was to evaluate the effect of eight aerobic and anaerobic oral commensal bacterial species on in vitro Candida albicans biofilm development. A single isolate of C. albicans 2560 g, and eight different species of oral bacteria comprising, Actinomyces israelii, Lactobacillus acidophilus, Prevotella nigrescens, Porphyromonas gingivalis, Pseudomonas aeruginosa, Escherichia coli, Streptococcus mutans, and Streptococcus intermedius were studied using an in vitro biofilm assay. Biofilm formation was quantified in terms of the ability of Candida to grow on polystyrene plastic surfaces co-cultured with the foregoing bacteria. A viable cell count was used to quantify the sessile yeast growth and scanning electron microscopy was employed to confirm and visualize biofilm formation. Co-culture with differing concentrations of bacteria had variable effects on Candida biofilm formation. Co-culture with the highest concentrations of each of the foregoing bacteria resulted in a consistent reduction in the yeast counts in the candidal biofilm (P < 0.05), except for L. acidophilus, S. mutans, and, S. intermedius co-cultures. Further, on regression analysis a significant negative correlation between the co-culture concentration of either P. gingivalis or E. coli and viable yeast counts in the biofilm was noted (P < 0.05) although this was not evident for the other bacterial species. Taken together, our data indicate that, quantitative and qualitative nature of the bacteria modulate C. albicans biofilm formation in mixed species environments such as the oral cavity.
Keyword Biofilm
Candida albicans
Oral bacteria
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 78 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 78 times in Scopus Article | Citations
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