Coaggregation profiles of the microflora from root surface caries lesions

Shen, S., Samaranayake, L. P. and Yip, H. -K. (2005) Coaggregation profiles of the microflora from root surface caries lesions. Archives of Oral Biology, 50 1: 23-32. doi:10.1016/j.archoralbio.2004.07.002


Author Shen, S.
Samaranayake, L. P.
Yip, H. -K.
Title Coaggregation profiles of the microflora from root surface caries lesions
Journal name Archives of Oral Biology   Check publisher's open access policy
ISSN 0003-9969
1879-1506
Publication date 2005-01-01
Year available 2004
Sub-type Article (original research)
DOI 10.1016/j.archoralbio.2004.07.002
Open Access Status Not yet assessed
Volume 50
Issue 1
Start page 23
End page 32
Total pages 10
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Formatted abstract
Bacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. As no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single Candida spp. The quantitative coaggregation assay we used proved to be a more sensitive method than the semi-quantitative, visual evaluation as the results yielded the percent coaggregation. Fusobacterium nucleatum, Lactobacillus acidophilus, Streptococcus bovis II/2 and Gemella morbillorum were observed having higher degrees of autoaggregation than the other examined strains. Significant levels of inter-species coaggregation was seen between: (1) Actinomyces spp. and Veillonella spp.; (2) Actinomyces israelii and Peptostreptococcus prevotii; (3) Campylobacter gracilis and Actinomyces spp.; (4) Prevotella intermedia and nine different species; and (5) Fusobacterium nucleatum and six other species. The single Candida albicans isolate did not coaggregate to a significant extent with any of the 21 bacterial isolates studied. Scanning electron microscopy observation of the coaggregation interactions between bacterial pairs having strong coaggregation reactions revealed varying adhesive patterns. Our findings on coaggregation amongst these isolates imply existence of multiple interactions between the coaggregation-inducing bacterial species in root caries. In particular, Actinomyces spp., Veillonella spp., Prevotella spp. and Fusobacterium spp. appear to play a significant role in this context.
Keyword Bacterial coaggregation
Biofilms
Dental plaque
Microflora
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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