Direct detection of Actinomyces spp. from infected root canals in a Chinese population: a study using PCR-based, oligonucleotide-DNA hybridization technique

Tang, Gaoyan, Samaranayake, Lakshman P., Yip, Hak-Kong, Chu, Frederick C. S., Tsang, Peter C. S. and Cheung, Becky P. K. (2003) Direct detection of Actinomyces spp. from infected root canals in a Chinese population: a study using PCR-based, oligonucleotide-DNA hybridization technique. Journal of Dentistry, 31 8: 559-568. doi:10.1016/S0300-5712(03)00112-X


Author Tang, Gaoyan
Samaranayake, Lakshman P.
Yip, Hak-Kong
Chu, Frederick C. S.
Tsang, Peter C. S.
Cheung, Becky P. K.
Title Direct detection of Actinomyces spp. from infected root canals in a Chinese population: a study using PCR-based, oligonucleotide-DNA hybridization technique
Journal name Journal of Dentistry   Check publisher's open access policy
ISSN 0300-5712
1879-176X
Publication date 2003-11-01
Year available 2003
Sub-type Article (original research)
DOI 10.1016/S0300-5712(03)00112-X
Open Access Status Not yet assessed
Volume 31
Issue 8
Start page 559
End page 568
Total pages 10
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
Objectives: The poor sensitivity of phenotypic identification techniques has hampered the taxonomic differentiation of Actinomyces. Hence we developed a sensitive and specific, PCR-based oligonucleotide-DNA hybridization technique to detect Actinomyces spp. and, used this method to detect these organisms in samples directly obtained from infected root canals.

Methods: A total of 32 samples from 28 Chinese patients, with primary root canal infections, aseptically exposed at the first patient visit, were studied. Whole bacterial genomic DNA was isolated directly from paper point samples. The variable regions of 16S ribosomal DNA of bacteria were amplified and labeled with digoxigenin for further hybridization and detection. A total of seven oligonucleotide probes specific for A. bovis, A. gerencseriae, A. israelii, A. meyeri, catalase-negative A. naeslundii (genospecies 1 and 2), catalase-positive A. naeslundii genospecies 2 and A. odontolyticus were used.

Results: 16 of the 32 teeth were infected with one or more Actinomyces species. The prevalence rates of the examined species were: A. odontolyticus 31.3%, A. meyeri 9.4%, A. naeslundii 9.4%, A. israelii 6.3% and A. gerencseriae 3.1%; no A. bovis was detected in any of the canals. Furthermore, A. odontolyticus was isolated more frequently from root canals with caries or a history of caries (Fisher's exact test: P=0.0496; Odds ratio=9.00, 95% confidence interval: 0.97-83.63), and A. naeslundii was significantly associated with traumatized teeth (Fisher's exact test: P=0.0121; Odds ratio=57.00, 95% confidence interval: 2.10-1546.90). However, no significant correlation was found between Actinomyces spp. and clinical symptoms and signs, such as pain, swelling, percussion to tenderness, sinus and periapical radiolucency.

Conclusion: Actinomyces spp. may be important pathogens of root canal infections. A. naeslundii in particular may be related with traumatized teeth. A. odontolyticus appears to be involved in infections related to caries, exposure of dentinal tubules during cavity preparation and/or leaking restoration, but further clarification with large samples is necessary.
Keyword Actinomycetes
Root canal infection
Polymerase chain reaction
Otigonucleotide-DNA hybridization
Chinese
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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