Genotypic diversity of oral Actinomyces naeslundii genospecies 1 and 2 in caries-active preschool children

Tang, G., Samaranayake, L. P. and Yip, H. -K. (2004) Genotypic diversity of oral Actinomyces naeslundii genospecies 1 and 2 in caries-active preschool children. Oral Microbiology and Immunology, 19 6: 371-378. doi:10.1111/j.1399-302x.2004.00171.x


Author Tang, G.
Samaranayake, L. P.
Yip, H. -K.
Title Genotypic diversity of oral Actinomyces naeslundii genospecies 1 and 2 in caries-active preschool children
Formatted title
Genotypic diversity of oral Actinomyces naeslundii genospecies 1 and 2 in caries-active preschool children
Journal name Oral Microbiology and Immunology   Check publisher's open access policy
ISSN 0902-0055
2041-1014
Publication date 2004-12-01
Sub-type Article (original research)
DOI 10.1111/j.1399-302x.2004.00171.x
Open Access Status Not yet assessed
Volume 19
Issue 6
Start page 371
End page 378
Total pages 8
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
A total of 991 isolates of Actinomyces naeslundii were obtained from sound approximal tooth sites in either caries-active (n = 35) or caries-free (n = 20) preschool children. From this group of isolates, 101 strains were chosen to study the genotypic diversity of A. naeslundii genospecies 1 (n = 30), catalase-positive (n = 30), and catalase-negative genospecies 2 (n = 41). Polymerase chain reaction-restriction fragment length polymorphism PCR-RPLP), with a pair of primers targeting the 16S ribosome RNA gene (16S rDNA), and Mn/I digestion together with randomly amplified polymorphic DNA (RAPD) with eight arbitrary, single 10-mer primers were performed to generate genetic profiles of selected Actinomyces isolates. The hierarchic relationships of genetic profiles were finally analyzed using computerized dendrograms. There was no significant difference in the prevalence rates and proportions of either genospecies 1 or 2 between the caries-free and caries-active groups, although a higher prevalence of genospecies 2 was noted in the total population. Dendrogram analyses of the 16S rDNA PCR-RFLP profiles revealed that all strains belonging to A. naeslundii genospecies 1 could be subgrouped into three genotypes (T7, T18, and T19), with a single predominant genotype, T18 (27/30). Catalase-positive strains for genospecies 2 fell into three subtypes (T4, T7, and T17), whereas the catalase-negative counterparts were distributed amongst 16 subtypes. No specific genotype was significantly associated with caries activity. We conclude that heterogeneous subgroups of A. naeslundii genospecies 1 and 2, particularly the latter, are the constituent flora of dental plaque in children and may contribute to the pathogenesis of childhood caries.
Keyword Actinomyces
Caries
Genetic diversity
Primary dentition
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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