The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its impact on the colonization traits of Candida glabrata

Samaranayake, Y. H., Yau, J. Y. Y., Thein, Z. M., Jayatilake, J. A. M. S., Yeung, K. W. S. and Samaranayake, L. P. (2010) The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its impact on the colonization traits of Candida glabrata. Medical Mycology, 48 5: 725-734. doi:10.3109/13693780903496583


Author Samaranayake, Y. H.
Yau, J. Y. Y.
Thein, Z. M.
Jayatilake, J. A. M. S.
Yeung, K. W. S.
Samaranayake, L. P.
Title The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its impact on the colonization traits of Candida glabrata
Journal name Medical Mycology   Check publisher's open access policy
ISSN 1369-3786
1460-2709
Publication date 2010-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.3109/13693780903496583
Open Access Status
Volume 48
Issue 5
Start page 725
End page 734
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Language eng
Subject 2725 Infectious Diseases
3400 Veterinary
Abstract The post-antifungal effect (PAFE) has been shown to affect Candida pathogenicity, but there is little information on either PAFE or its association with the colonization traits of Candida glabrata. The objective of this study was to determine, in vitro, the PAFE on 14 C. glabrata isolates following exposure to amphotericin B (AMB), nystatin (NYS), ketoconazole (KETO) and 5-fluorocytosine (5FC). In addition, we evaluated the impact of PAFE on yeast adherence to buccal epithelial cells (BEC), cell-surface-hydrophobicity (CSH) and biofilm growth (BG) on denture acrylic surfaces. PAFE was induced following a 1-h exposure of yeasts to (×1×4MIC) of AMB, NYS, KETO and 5FC in RPMI medium and, measured using automated turbidometry. The BEC adhesion, CSH and BG assays were performed by the methods of Kimura & Pearsall, Sweet et al., and Jin et al., respectively. Significant differences in PAFE (P < 0.001) were observed after exposure to AMB and NYS, but not KETO and 5FC. Following exposure to AMB, NYS, KETO and 5FC, significant inter-strain differences (P < 0.001) were observed in percentage terms in adhesion (39.0%, 43.48%, 38.28%, 35.07%) and biofilm growth (42.86%, 39.86%, 42.81%, 36.38%), respectively. Short exposure of C. glabrata to sub-cidal concentrations of antifungals modulates yeast growth and also affects some of their colonization traits.
Keyword Antifungals
Candida glabrata
Colonization
PAFE
Virulence
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID 10205959
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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