Expression of human β-defensins-1 and -2 peptides in unresolved chronic periodontitis

Lu, Qian, Jin, Lijian, Darveau, Richard P. and Samaranayake, Lakshman P. (2004) Expression of human β-defensins-1 and -2 peptides in unresolved chronic periodontitis. Journal of Periodontal Research, 39 4: 221-227. doi:10.1111/j.1600-0765.2004.00727.x

Author Lu, Qian
Jin, Lijian
Darveau, Richard P.
Samaranayake, Lakshman P.
Title Expression of human β-defensins-1 and -2 peptides in unresolved chronic periodontitis
Journal name Journal of Periodontal Research   Check publisher's open access policy
ISSN 0022-3484
Publication date 2004-08-01
Sub-type Article (original research)
DOI 10.1111/j.1600-0765.2004.00727.x
Open Access Status Not yet assessed
Volume 39
Issue 4
Start page 221
End page 227
Total pages 7
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Background: Human β-defensins (hBDs) are antimicrobial peptides which contribute to host innate immunity by disrupting the membrane integrity of a broad spectrum of microorganisms. Objectives: This study aimed to determine the expression profiles of hBD-1 and -2 peptides in gingiva and to assess the possible relations of these antimicrobial peptides with periodontal health and disease.

Methods: Seven periodontally healthy subjects and 22 patients with unresolved chronic periodontitis were recruited and the gingival biopsies collected consisted of healthy tissues from the healthy subjects (HT-C); periodontal pocket tissues (PoT) and inflamed connective tissues (ICT) from the base of pocket, i.e. granulation tissues, as well as clinically healthy tissues (HT-P) from the adjacent clinically healthy sites from the patients. The expression of hBD-1 and -2 peptides was detected by immunohistochemistry and quantitatively analyzed with a computerized image processing system.

Results: Both hBD-1 and -2 peptides were detected in all periodontally healthy subjects, while hBD-1 was detected in all patients and hBD-2 was found in most of the patients. Their expression was mainly confined to the granular and spinous layers of gingival epithelium, in which hBD-1 was detected in both intercellular spaces and cytoplasm, whereas hBD-2 was mainly observed in the cytoplasm. HT-C expressed significantly higher levels of hBD-2 than HT-P (p < 0.05). Within the patients, both defensins were up-regulated significantly in PoT as compared with the adjacent HT-P (p < 0.05).

Conclusions: The present study showed that hBD-1 and -2 were frequently expressed in the granular and spinous layers of gingival epithelia and their expression may be associated with periodontal health and disease.
Keyword Chronic periodontitis
Gingival epithelium
Human β-defensins
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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