Expression of human β-defensin-3 in gingival epithelia

Lu, Qian, Samaranayake, Lakshman P., Darveau, Richard P. and Jin, Lijian (2005) Expression of human β-defensin-3 in gingival epithelia. Journal of Periodontal Research, 40 6: 474-481. doi:10.1111/j.1600-0765.2005.00827.x


Author Lu, Qian
Samaranayake, Lakshman P.
Darveau, Richard P.
Jin, Lijian
Title Expression of human β-defensin-3 in gingival epithelia
Formatted title
Expression of human β-defensin-3 in gingival epithelia
Journal name Journal of Periodontal Research   Check publisher's open access policy
ISSN 0022-3484
1600-0765
Publication date 2005-12-01
Sub-type Article (original research)
DOI 10.1111/j.1600-0765.2005.00827.x
Open Access Status Not yet assessed
Volume 40
Issue 6
Start page 474
End page 481
Total pages 8
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Objective: This study aimed to investigate the expression patterns of the newly discovered human β-defensin-3 (hBD-3) in human gingiva.

Background: Human β-defensins (hBDs) are a group of small, broad-spectrum, cationic antimicrobial peptides. Our recent study showed that the expression levels of hBD-1 and 2 peptides were associated with periodontal conditions.

Methods: A total of 49 gingival biopsies were collected, including 33 samples from 21 patients with chronic periodontitis and 16 samples from 16 periodontally healthy subjects. The expression of hBD-3 was detected by immunohistochemistry and in situ hybridization. Double staining was undertaken to identify hBD-3 peptide-positive cells, using CD-1a and cytokeratin 20 as markers for Langerhans cells and Merkel cells, respectively.

Results: hBD-3 peptide was detected in 88% of the samples, which was confined to the gingival epithelia. In healthy control subjects, hBD-3 peptide was more frequently detected in the basal layer as compared to the patients (53% vs. 18%, p < 0.05). In patients, hBD-3 expression extended from the basal layer to the spinous layers (82%), in which hBD-3 was confined to the basal and deep spinous layers in clinically healthy tissues from patients, whereas it extended to the superficial spinous layers in pocket tissues from patients (0% vs. 50%, p < 0.05). In both groups, hBD-3 peptide was expressed not only in gingival keratinocytes, but also in Langerhans cells and Merkel cells. hBD-3 transcripts were detected in 90% of the samples and they were confined to the basal and/or suprabasal layers of gingival epithelia.

Conclusions: This study shows that hBD-3 is frequently expressed in gingival epithelia. The appropriate expression of hBD-3 peptide may contribute to the maintenance of periodontal homeostasis, possibly through its antimicrobial effect and promotion of adaptive immune responses.
Keyword Gingival epithelia
Human β-defensin-3
Immunohistochemistry
In situ hybridization
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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