Exploring the oral bacterial flora: current status and future directions

Parahitiyawa, N. B., Scully, C., Leung, W. K., Yam, W. C., Jin, L. J. and Samaranayake, L. P. (2010) Exploring the oral bacterial flora: current status and future directions. Oral Diseases, 16 2: 136-145. doi:10.1111/j.1601-0825.2009.01607.x

Author Parahitiyawa, N. B.
Scully, C.
Leung, W. K.
Yam, W. C.
Jin, L. J.
Samaranayake, L. P.
Title Exploring the oral bacterial flora: current status and future directions
Journal name Oral Diseases   Check publisher's open access policy
ISSN 1354-523X
Publication date 2010-01-01
Year available 2010
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1111/j.1601-0825.2009.01607.x
Open Access Status Not yet assessed
Volume 16
Issue 2
Start page 136
End page 145
Total pages 10
Place of publication Malden, MA, United States
Publisher Wiley-Blackwell
Language eng
Formatted abstract
Objective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods.

Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed.

Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised.

Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species.

Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches. 
Keyword Immunology and microbiology
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID RGC HKU 7624/06M
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: School of Dentistry Publications
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