Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development

Bandara, H. M. H. N., Yau, J. Y. Y., Watt, R. M., Jin, L. J. and Samaranayake, L. P. (2010) Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development. BMC Microbiology, 10 125.1-125.9. doi:10.1186/1471-2180-10-125

Author Bandara, H. M. H. N.
Yau, J. Y. Y.
Watt, R. M.
Jin, L. J.
Samaranayake, L. P.
Title Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development
Formatted title
Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development
Journal name BMC Microbiology   Check publisher's open access policy
ISSN 1471-2180
Publication date 2010-04-25
Year available 2010
Sub-type Article (original research)
DOI 10.1186/1471-2180-10-125
Open Access Status DOI
Volume 10
Start page 125.1
End page 125.9
Total pages 9
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Subject 2726 Microbiology (medical)
Formatted abstract
Background: Elucidation of the communal behavior of microbes in mixed species biofilms may have a major impact on understanding infectious diseases and for the therapeutics. Although, the structure and the properties of monospecies biofilms and their role in disease have been extensively studied during the last decade, the interactions within mixed biofilms consisting of bacteria and fungi such as Candida spp. have not been illustrated in depth. Hence, the aim of this study was to evaluate the interspecies interactions of Pseudomonas aeruginosa and six different species of Candida comprising C. albicans, C. glabrata, C. krusei, C. tropicalis, C. parapsilosis, and C. dubliniensis in dual species biofilm development.

Results: A significant reduction in colony forming units (CFU) of C. parapsilosis (90 min), C. albicans and C. tropicalis (90 min, 24h and 48h), C. dubliniensis and C. glabrata, (24h and 48h) was noted when co-cultured with P. aeruginosa in comparison to their monospecies counterparts (P<0.05). A simultaneous significant reduction in P. aeruginosa numbers grown with C. albicans (90 min and 48h), C. krusei (90 min, 24h and 48h), C. glabrata, (24 h and 48h), and an elevation of P. aeruginosa numbers co-cultured with C. tropicalis (48 h) was noted (P<0.05). When data from all Candida spp. and P. aeruginosa were pooled, highly significant mutual inhibition of biofilm formation was noted (Candida P<0.001, P. aeruginosa P<0.01). Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM) analyses confirmed scanty architecture in dual species biofilm in spite of dense colonization in monospecies counterparts.

Conclusions: P. aeruginosa and Candida in a dual species environment mutually suppress biofilm development, both quantitatively and qualitatively. These findings provide a foundation to clarify the molecular basis of bacterial-fungal interactions, and to understand the pathobiology of mixed bacterial-fungal infections.
Keyword Microbiology
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID CERG HKU 7624/06M
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 45 times in Thomson Reuters Web of Science Article | Citations
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