Characterization of switch phenotypes in Candida albicans biofilms

Jin, Y., Samaranayake, Y. H., Yip, H. K. and Samaranayake, L. P. (2005) Characterization of switch phenotypes in Candida albicans biofilms. Mycopathologia, 160 3: 191-200. doi:10.1007/s11046-005-6331-x

Author Jin, Y.
Samaranayake, Y. H.
Yip, H. K.
Samaranayake, L. P.
Title Characterization of switch phenotypes in Candida albicans biofilms
Formatted title
Characterization of switch phenotypes in Candida albicans biofilms
Journal name Mycopathologia   Check publisher's open access policy
ISSN 0301-486X
Publication date 2005-10-01
Year available 2005
Sub-type Article (original research)
DOI 10.1007/s11046-005-6331-x
Open Access Status Not yet assessed
Volume 160
Issue 3
Start page 191
End page 200
Total pages 10
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Formatted abstract
The aim of this study was to characterize switch phenotypes in Candida albicans biofilms. Cells of Candida albicans 192887g biofilms (24 h) were resuspended and these together with their planktonic counterparts were separately inoculated on Lee's medium agar supplemented with arginine and zinc, at 25°C for 9 days, for colony formation. The different switch phenotypes, as reflected by varying colony morphologies, were then examined for their (i) stability under various growth conditions, (ii) carbohydrate assimilation profiles, (iii) susceptibility to the polyene antifungal, nystatin, (iv) adhering and biofilm-forming ability, (v) filamentation, and (vi) growth rate in yeast nitrogen base medium supplemented with 100 mM glucose. Our data showed that the frequency of phenotypic switching in C. albicans biofilms was approximately 1%. Compared with the planktonic yeasts, cells derived from candidal biofilms generated one of the phenotypes less frequently (Chi-square-tests: P = 0.017). The five phenotypes derived from the biofilm growth demonstrated differing profiles for carbohydrate assimilation, adhesion, biofilm formation, filamentation, and growth rate. These findings reported here, for the first time, imply that phenotypic switching in the candidal biofilms differs from that in the planktonic growth, and affects multiple biological attributes.
Keyword Biofilm
Candida albicans
Phenotypic switching
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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