Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva

Ajonuma, Louise C., Lu, Qian, Cheung, Becky P. K., Leung, W. Keung, Samaranayake, Lakshman P. and Jin, Lijian (2010) Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva. Cell Biology International, 34 2: 147-152. doi:10.1042/CBI20090019


Author Ajonuma, Louise C.
Lu, Qian
Cheung, Becky P. K.
Leung, W. Keung
Samaranayake, Lakshman P.
Jin, Lijian
Title Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva
Journal name Cell Biology International   Check publisher's open access policy
ISSN 1065-6995
1095-8355
Publication date 2010-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1042/CBI20090019
Volume 34
Issue 2
Start page 147
End page 152
Total pages 6
Place of publication Oxford, United Kingdom
Publisher John Wiley and Sons Ltd.
Language eng
Subject 1307 Cell Biology
Abstract CFTR (cystic fibrosis transmembrane conductance regulator) is a cAMP-activated chloride channel that regulates electrolyte and water transport. The present study investigated the expression and localization of CFTR in human gingiva and explored the possible association of CFTR with periodontal conditions. CFTR expression in gingival biopsies from five periodontally healthy subjects and ten subjects with chronic periodontitis and in the RHGE (reconstituted human gingival epithelia) was detected by immunohistochemistry, whereas its expression in gingival biopsies was analysed by immunofluorescence staining. CFTR mRNA was analysed by reverse transcription-PCR. CFTR mRNA was detected in human gingival epithelia and RHGE. CFTR protein was detected in gingival biopsies from both healthy subjects and individuals with periodontitis and in RHGE. In healthy subjects, CFTR expression was mainly confined to the granular and spinous layers of epithelia and localized on the cell membrane. In patients with periodontitis, CFTR was detected in all layers of epithelia and the underlying connective tissues. The mean CFTR expression levels in periodontitis patients were significantly higher than those in healthy subjects. The present study for the first time showed the expression and localization of CFTR in human gingival epithelia. Elevated CFTR expression in periodontitis subjects implies the possible involvement of CFTR in periodontal disease pathogenesis. Further study is warranted to confirm the present findings. © The Author(s) Journal compilation
Keyword Gene expression
Gingival epithelium
Periodontal disease
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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