Non-steroidal anti-inflammatory drugs may modulate the protease activity of candida albicans

Carvalho, Alessandra P., Gursky,Lauren C., Rosa, Rosimeire T., Rymovicz, Alinne U. M., Campelo, Patricia M. S., Gregio, Ana Maria T., Koga-Ito, Cristiane Y., Samaranayake, Lakshman P. and Rosa, Edvaldo A. R. (2010) Non-steroidal anti-inflammatory drugs may modulate the protease activity of candida albicans. Microbial Pathogenesis, 49 6: 315-322. doi:10.1016/j.micpath.2010.07.007

Author Carvalho, Alessandra P.
Gursky,Lauren C.
Rosa, Rosimeire T.
Rymovicz, Alinne U. M.
Campelo, Patricia M. S.
Gregio, Ana Maria T.
Koga-Ito, Cristiane Y.
Samaranayake, Lakshman P.
Rosa, Edvaldo A. R.
Title Non-steroidal anti-inflammatory drugs may modulate the protease activity of candida albicans
Journal name Microbial Pathogenesis   Check publisher's open access policy
ISSN 0882-4010
Publication date 2010-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.micpath.2010.07.007
Volume 49
Issue 6
Start page 315
End page 322
Total pages 8
Place of publication Camden, London, United Kingdom
Publisher Academic Press
Language eng
Subject 2404 Microbiology
2725 Infectious Diseases
Abstract The phenotypic pressure exerted by non-steroidal anti-inflammatory drugs (NSAIDs) on autochthonous and pathogenic microbiota remains sparsely known. In this study, we investigated if some NSAIDs increment or diminish the secretion of aspartyl-proteases (Sap) by Candida albicans grown under different phenotypes and oxygen availability using a set of SAP knock-out mutants and other set for genes (EFG1 and CPH1) that codify transcription factors involved in filamentation and protease secretion. Pre-conditioned cells were grown under planktonic and biofilm phenotypes, in normoxia and anoxia, in the presence of plasma concentrations of acetylsalicylic acid, diclofenac, indomethacin, nimesulide, piroxicam, ibuprofen, and acetaminophen. For diclofenac, indomethacin, nimesulide, and piroxicam the secretion rates of Sap by SAP1-6, EFG1, and CPH1 mutants were similar or, even, inferior to parental wild-type strain. This suggests that neither Sap 1-6 isoenzymes nor Efg1/Cph1 pathways may be entirely responsible for protease release when exposed to these NSAIDs. Ibuprofen and acetaminophen enhanced Sap secretion rates in three environmental conditions (normoxic biofilm, normoxic planktonic and anoxic planktonic). In other hand, aspirin seems to reduce the Sap-related pathogenic behavior of candidal biofilms. Modulation of Sap activity may occur according to candidal phenotypic state, oxygen availability, and type of NSAID to which the cells are exposed.
Keyword Anoxia
Candida albicans
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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