Bioactivity and architecture of Candida albicans biofilms developed on poly(methyl methacrylate) resin surface

Da Silva, Wander Jose, Seneviratne, Jayampath, Samaranayake, Lakshman Perera and Del Bel Cury, Altair Antoninha (2010) Bioactivity and architecture of Candida albicans biofilms developed on poly(methyl methacrylate) resin surface. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 94 1: 149-156. doi:10.1002/jbm.b.31635


Author Da Silva, Wander Jose
Seneviratne, Jayampath
Samaranayake, Lakshman Perera
Del Bel Cury, Altair Antoninha
Title Bioactivity and architecture of Candida albicans biofilms developed on poly(methyl methacrylate) resin surface
Journal name Journal of Biomedical Materials Research - Part B Applied Biomaterials   Check publisher's open access policy
ISSN 1552-4973
1552-4981
Publication date 2010-01-01
Year available 2010
Sub-type Article (original research)
DOI 10.1002/jbm.b.31635
Volume 94
Issue 1
Start page 149
End page 156
Total pages 8
Place of publication Hoboken, NJ United States
Publisher John Wiley and Sons, Inc.
Language eng
Subject 2204 Religion and Religious Studies
2502 Biomaterials
Abstract The aim of this study was to evaluate the bioactivity and architecture of Candida albicans biofilms developed on the surface of poly(methyl methacrylate) (PMMA) resin. To do this, surface roughness (SR) and surface free energy of PMMA specimens were measured. Next, the biofilms of two different C. albicans strains (ATCC 90028 and SC5314) were allowed to develop on the PMMA surface and evaluated at 24, 48, and 72 h after adhesion. The bioactivity of the biofilms was measured by the XTT reduction assay. Biofilm topography was evaluated by scanning electron microscopy. Confocal microscopy was used to evaluate the architectural properties of bio-volume, average thickness, biofilm roughness, surface area/volume ratio and the proportion of live/dead cells in the different biofilm development stages. SR and SFE had no influence on biofilm development. Each strain exhibited a different biofilm activity (P < 0.001). Confocal images showed different architectures for the different biofilm development stages. We conclude that the main differences detected in biofilm bioactivity and architecture were related to the characteristics of each C. albicans strain and to biofilm development time.
Keyword Adhesion
Biofilm
Candida albicans
Poly(methyl methacrylate)
Surface properties
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
 
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