An outbreak of Potato spindle tuber viroid in tomato is linked to imported seed

van Brunschot, S. L., Verhoeven, J. Th, J., Persley, D. M., Geering, A. D. W., Drenth, A. and Thomas, J. E. (2014) An outbreak of Potato spindle tuber viroid in tomato is linked to imported seed. European Journal of Plant Pathology, 139 1: 1-7. doi:10.1007/s10658-014-0379-8


Author van Brunschot, S. L.
Verhoeven, J. Th, J.
Persley, D. M.
Geering, A. D. W.
Drenth, A.
Thomas, J. E.
Title An outbreak of Potato spindle tuber viroid in tomato is linked to imported seed
Journal name European Journal of Plant Pathology   Check publisher's open access policy
ISSN 0929-1873
1573-8469
Publication date 2014-01-19
Year available 2014
Sub-type Article (original research)
DOI 10.1007/s10658-014-0379-8
Volume 139
Issue 1
Start page 1
End page 7
Total pages 7
Place of publication Dordrecht, Netherlands
Publisher Springer
Language eng
Formatted abstract
In 2011, an outbreak of the quarantine-regulated pathogen Potato spindle tuber viroid (PSTVd) occurred in a commercial glasshouse-grown tomato crop in Queensland, Australia. Phylogenetic studies showed that the genotype of this isolate grouped in a cluster of PSTVd genotypes from tomato and Physalis peruviana, and exhibited an interesting mutation (U257→A) that has previously been linked to lethal symptom expression in tomato. Transmission studies showed that the viroid could be mechanically transmitted from crushed fruit sap, but not from undamaged fruits. A low rate of asymptomatic infection was determined for plants in the affected glasshouse, demonstrating the efficacy of using symptoms to detect PSTVd infections in tomato. No PSTVd infections were detected in solanaceous weeds located outside of the infected glasshouse, excluding them from playing a role in the viroid epidemiology. Monitoring and subsequent testing of new tomato crops grown in the facility demonstrated successful eradication of the pathogen. A trace-back analysis linked the outbreak of PSTVd to an infected imported tomato seed-lot, indicating that PSTVd is transmitted internationally through contaminated seed.
Keyword Pospiviroid
Solanum lycopersicum
Quarantine
Inoculation
Detection
RT-PCR
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Published online ahad of print 19 January 2014.

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 7 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 03 Feb 2014, 22:29:53 EST by Sharon Van Brunschot on behalf of School of Biological Sciences