A bead-based suspension array for the multiplexed detection of begomoviruses and their whitefly vectors

van Brunschot, S. L., Bergervoet, J. H. W., Pagendam, D. E., de Weerdt, M., Geering, A. D. W., Drenth, A. and van der Vlugt, R. A. A. (2014) A bead-based suspension array for the multiplexed detection of begomoviruses and their whitefly vectors. Journal of Virological Methods, 198 86-94. doi:10.1016/j.jviromet.2013.12.014

Author van Brunschot, S. L.
Bergervoet, J. H. W.
Pagendam, D. E.
de Weerdt, M.
Geering, A. D. W.
Drenth, A.
van der Vlugt, R. A. A.
Title A bead-based suspension array for the multiplexed detection of begomoviruses and their whitefly vectors
Journal name Journal of Virological Methods   Check publisher's open access policy
ISSN 0166-0934
Publication date 2014-03-15
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.jviromet.2013.12.014
Volume 198
Start page 86
End page 94
Total pages 9
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Formatted abstract
• A bead-based array that detects plant viruses and their insect vectors is described.
• The multiplexed array was shown to be highly specific, sensitive and reproducible.
• Comparison with qPCR showed equal sensitivity of both methods.
• The array may facilitate the improved management of invasive pests and diseases.

Bead-based suspension array systems enable simultaneous fluorescence-based identification of multiple nucleic acid targets in a single reaction. This study describes the development of a novel approach to plant virus and vector diagnostics, a multiplexed 7-plex array that comprises a hierarchical set of assays for the simultaneous detection of begomoviruses and Bemisia tabaci, from both plant and whitefly samples. The multiplexed array incorporates genus, species and strain-specific assays, offering a unique approach for identifying both known and unknown viruses and B. tabaci species. When tested against a large panel of sequence-characterized begomovirus and whitefly samples, the array was shown to be 100% specific to the homologous target. Additionally, the multiplexed array was highly sensitive, efficiently and concurrently determining both virus and whitefly identity from single viruliferous whitefly samples. The detection limit for one assay within the multiplexed array that specifically detects Tomato yellow leaf curl virus-Israel (TYLCV-IL) was quantified as 200 fg of TYLCV-IL DNA, directly equivalent to that of TYLCV-specific qPCR. Highly reproducible results were obtained over multiple tests. The flexible multiplexed array described in this study has great potential for use in plant quarantine, biosecurity and disease management programs worldwide.
Keyword Luminex
Bemisia tabaci
Tomato yellow leaf curl virus
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Available online 31 December 2013

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
Official 2014 Collection
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 7 times in Scopus Article | Citations
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Created: Mon, 03 Feb 2014, 22:25:32 EST by Sharon Van Brunschot on behalf of School of Agriculture and Food Sciences