Development of a multiplexed bead-based suspension array for the detection and discrimination of Pospiviroid plant pathogens

van Brunschot, Sharon L., Bergervoet, Jan H. W., Pagendam, Daniel E., de Weerdt, Marjanne, Geering, Andrew D. W., Drenth, André and van der Vlugt, René A. A. (2014) Development of a multiplexed bead-based suspension array for the detection and discrimination of Pospiviroid plant pathogens. PLoS One, 9 1: e84743.1-e84743.12. doi:10.1371/journal.pone.0084743


Author van Brunschot, Sharon L.
Bergervoet, Jan H. W.
Pagendam, Daniel E.
de Weerdt, Marjanne
Geering, Andrew D. W.
Drenth, André
van der Vlugt, René A. A.
Title Development of a multiplexed bead-based suspension array for the detection and discrimination of Pospiviroid plant pathogens
Formatted title
Development of a multiplexed bead-based suspension array for the detection and discrimination of Pospiviroid plant pathogens
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2014-01-03
Sub-type Article (original research)
DOI 10.1371/journal.pone.0084743
Open Access Status DOI
Volume 9
Issue 1
Start page e84743.1
End page e84743.12
Total pages 12
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Abstract Efficient and reliable diagnostic tools for the routine indexing and certification of clean propagating material are essential for the management of pospiviroid diseases in horticultural crops. This study describes the development of a true multiplexed diagnostic method for the detection and identification of all nine currently recognized pospiviroid species in one assay using Luminex bead-based suspension array technology. In addition, a new data-driven, statistical method is presented for establishing thresholds for positivity for individual assays within multiplexed arrays. When applied to the multiplexed array data generated in this study, the new method was shown to have better control of false positives and false negative results than two other commonly used approaches for setting thresholds. The 11-plex Luminex MagPlex-TAG pospiviroid array described here has a unique hierarchical assay design, incorporating a near-universal assay in addition to nine species-specific assays, and a co-amplified plant internal control assay for quality assurance purposes. All assays of the multiplexed array were shown to be 100% specific, sensitive and reproducible. The multiplexed array described herein is robust, easy to use, displays unambiguous results and has strong potential for use in routine pospiviroid indexing to improve disease management strategies.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Queensland Alliance for Agriculture and Food Innovation
Official 2015 Collection
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 12 times in Scopus Article | Citations
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Created: Mon, 03 Feb 2014, 22:06:33 EST by Sharon Van Brunschot on behalf of School of Biological Sciences