Measurement of dynamic F-actin changes during exocytosis

Thorn, Peter (2014). Measurement of dynamic F-actin changes during exocytosis. In Andrea I. Ivanov (Ed.), Exocytosis and endocytosis 2nd ed. (pp. 423-431) New York, United States: Springer. doi:10.1007/978-1-4939-0944-5_29

Author Thorn, Peter
Title of chapter Measurement of dynamic F-actin changes during exocytosis
Title of book Exocytosis and endocytosis
Place of Publication New York, United States
Publisher Springer
Publication Year 2014
Sub-type Chapter in reference work, encyclopaedia, manual or handbook
DOI 10.1007/978-1-4939-0944-5_29
Open Access Status Not Open Access
Series Methods in molecular biology
Edition 2nd
ISBN 9781493909438
ISSN 1064-3745
Editor Andrea I. Ivanov
Volume number 1174
Chapter number 29
Start page 423
End page 431
Total pages 9
Language eng
Formatted Abstract/Summary
Exocytosis requires the fusion of vesicle membrane to the cell membrane. It is tightly regulated and orchestrated in space and time by diverse cellular mechanisms. It has long been recognized that one of these mechanisms is an essential role played by the cytoskeleton. In particular, accumulating evidence shows that the F-actin network is engaged during the final stages of vesicle interactions with the cell membrane. Using a combination of methods it is now possible to gain insights into F-actin dynamics and reveal its role during exocytosis. Here, we describe the use of two-photon and confocal microscopy to visualize F-actin changes at the cell membrane during exocytosis.
Keyword F-actin
Q-Index Code BX
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Book Chapter
Collection: School of Biomedical Sciences Publications
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Created: Sat, 01 Feb 2014, 02:06:20 EST by Assoc Prof Peter Thorn on behalf of School of Biomedical Sciences