The effect of gestational age on angiogenic gene expression in the rat placenta

Vaswani, Kanchan, Hum, Melissa Wen-Ching, Chan, Hsiu-Wen, Ryan, Jennifer, Wood-Bradley, Ryan J., Nitert, Marloes Dekker, Mitchell, Murray D., Armitage, James A. and Rice, Gregory E. (2013) The effect of gestational age on angiogenic gene expression in the rat placenta. PloS One, 8 12: . doi:10.1371/journal.pone.0083762

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Author Vaswani, Kanchan
Hum, Melissa Wen-Ching
Chan, Hsiu-Wen
Ryan, Jennifer
Wood-Bradley, Ryan J.
Nitert, Marloes Dekker
Mitchell, Murray D.
Armitage, James A.
Rice, Gregory E.
Title The effect of gestational age on angiogenic gene expression in the rat placenta
Journal name PloS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2013-12-31
Year available 2013
Sub-type Article (original research)
DOI 10.1371/journal.pone.0083762
Open Access Status DOI
Volume 8
Issue 12
Total pages 11
Place of publication San Francisco, United States
Publisher Public Library of Science
Language eng
Formatted abstract
The placenta plays a central role in determining the outcome of pregnancy. It undergoes changes during gestation as the fetus develops and as demands for energy substrate transfer and gas exchange increase. The molecular mechanisms that coordinate these changes have yet to be fully elucidated. The study performed a large scale screen of the transcriptome of the rat placenta throughout mid-late gestation (E14.25–E20) with emphasis on characterizing gestational age associated changes in the expression of genes invoved in angiogenic pathways. Sprague Dawley dams were sacrificed at E14.25, E15.25, E17.25 and E20 (n = 6 per group) and RNA was isolated from one placenta per dam. Changes in placental gene expression were identifed using Illumina Rat Ref-12 Expression BeadChip Microarrays. Differentially expressed genes (>2-fold change, <1% false discovery rate, FDR) were functionally categorised by gene ontology pathway analysis. A subset of differentially expressed genes identified by microarrays were confirmed using Real-Time qPCR. The expression of thirty one genes involved in the angiogenic pathway was shown to change over time, using microarray analysis (22 genes displayed increased and 9 gene decreased expression). Five genes (4 up regulated: Cd36, Mmp14, Rhob and Angpt4 and 1 down regulated: Foxm1) involved in angiogenesis and blood vessel morphogenesis were subjected to further validation. qPCR confirmed late gestational increased expression of Cd36, Mmp14, Rhob and Angpt4 and a decrease in expression of Foxm1 before labour onset (P<0.0001). The observed acute, pre-labour changes in the expression of the 31 genes during gestation warrant further investigation to elucidate their role in pregnancy.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes Article number e83762.

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2014 Collection
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
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Created: Fri, 31 Jan 2014, 19:46:36 EST by Roheen Gill on behalf of UQ Centre for Clinical Research