Validity of the Enzyme-linked Immunoelectrotransfer Blot (EITB) for naturally acquired porcine cysticercosis

Jayashi, César M., Gonzalez, Armando E., Castillo Neyra, Ricardo, Rodríguez, Silvia, García, Rodríguez, Lightowlers, Marshall W. and The Cysticercosis Working Group in Peru (2014) Validity of the Enzyme-linked Immunoelectrotransfer Blot (EITB) for naturally acquired porcine cysticercosis. Veterinary Parasitology, 199 1-2: 42-49. doi:10.1016/j.vetpar.2013.10.004


Author Jayashi, César M.
Gonzalez, Armando E.
Castillo Neyra, Ricardo
Rodríguez, Silvia
García, Rodríguez
Lightowlers, Marshall W.
The Cysticercosis Working Group in Peru
Title Validity of the Enzyme-linked Immunoelectrotransfer Blot (EITB) for naturally acquired porcine cysticercosis
Journal name Veterinary Parasitology   Check publisher's open access policy
ISSN 0304-4017
1873-2550
Publication date 2014-01-17
Year available 2013
Sub-type Article (original research)
DOI 10.1016/j.vetpar.2013.10.004
Open Access Status DOI
Volume 199
Issue 1-2
Start page 42
End page 49
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 2405 Parasitology
3400 Veterinary
Formatted abstract
The Enzyme-linked Immunoelectrotransfer Blot (EITB) has been used widely as a screening test for Taenia solium cysticercosis in swine. However, the relation between seropositivity and infection in pig populations from endemic areas has not been well defined. The aim of this study is to relate EITB seropositivity with infection and infection burden, analyse the trade-off between sensitivity and specificity with various cut-off points for the EITB assay, and finally describe the serology changes in a cohort of rural pigs raised under natural conditions. A group of 107 pigs that were used as controls during a vaccination field trial in Peru was our study population. The prevalence of porcine cysticercosis determined by necropsy examination was 16.82% (18/107) in these animals. Using EITB reactivity to ≥1 band as a cut-off point for the assay, the sensitivity was 88.89% (65.29-98.62, 95% CI) and the specificity was 48.31% (37.59-59.16, 95% CI). Comparing other cut-off points, involving up to as many as 7 reactive bands, a reactivity of ≥3 bands provided the best trade-offs in sensitivity and specificity. Using this cut-off point for the assay, the sensitivity was 77.77% (52.36-93.59, 95% CI) and the specificity was 76.40% (66.22-84.76, 95% CI). A significant association was found between cyst counts over 100 cysts and reactivity to ≥3 bands in the EITB assay (Fisher's exact test, p< 0.05). The results of this study suggest that the use of the EITB assay to study porcine cysticercosis may require setting different cut-offs under field and experimental conditions, and depending upon the objective of the screening process.
Keyword Enzyme-linked immunoelectrotransfer blot (EITB) assay
Porcine cysticercosis
Receiver operating characteristic (ROC) curve
Sensitivity
Specificity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Available online 16 October 2013

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Thu, 30 Jan 2014, 03:02:38 EST by Cesar Jayashi Flores on behalf of Aust Institute for Bioengineering & Nanotechnology