The characterization of sponge NLRs provides insight into the origin and evolution of this innate immune gene family in animals

Yuen, Benedict, Bayes, Joanne M. and Degnan, Sandie M. (2014) The characterization of sponge NLRs provides insight into the origin and evolution of this innate immune gene family in animals. Molecular Biology and Evolution, 31 1: 106-120. doi:10.1093/molbev/mst174


Author Yuen, Benedict
Bayes, Joanne M.
Degnan, Sandie M.
Title The characterization of sponge NLRs provides insight into the origin and evolution of this innate immune gene family in animals
Journal name Molecular Biology and Evolution   Check publisher's open access policy
ISSN 0737-4038
1537-1719
Publication date 2014-01-01
Year available 2013
Sub-type Article (original research)
DOI 10.1093/molbev/mst174
Volume 31
Issue 1
Start page 106
End page 120
Total pages 15
Place of publication Cary, NC, United States
Publisher Oxford University Press
Language eng
Formatted abstract
The "Nucleotide-binding domain and Leucine-rich Repeat" (NLR) genes are a family of intracellular pattern recognition receptors (PRR) that are a critical component of the metazoan innate immune system, involved in both defense against pathogenic microorganisms and in beneficial interactions with symbionts. To investigate the origin and evolution of the NLR gene family, we characterized the full NACHT domain-containing gene complement in the genome of the sponge, Amphimedon queenslandica. As sister group to all animals, sponges are ideally placed to inform our understanding of the early evolution of this ancient PRR family. Amphimedon queenslandica has a large NACHT domain-containing gene complement that is dominated by bona fide NLRs (n = 135) with varied phylogenetic histories. Approximately half of these have a tripartite architecture that includes an N-terminal CARD or DEATH domain. The multiplicity of the A. queenslandica NLR genes and the high variability across the N- and C-terminal domains are consistent with involvement in immunity. We also provide new insight into the evolution of NLRs in invertebrates through comparative genomic analysis of multiple metazoan and nonmetazoan taxa. Specifically, we demonstrate that the NLR gene family appears to be a metazoan innovation, characterized by two major gene lineages that may have originated with the last common eumetazoan ancestor. Subsequent lineage-specific gene duplication, gene loss and domain shuffling all have played an important role in the highly dynamic evolutionary history of invertebrate NLRs.
Keyword Innate immunity
Invertebrate genomics
NACHT domain
Porifera
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes First published online: 3 October 2013.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2014 Collection
School of Biological Sciences Publications
 
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