Characterization of the Polyubiquitin Gene in the Marine Red Alga Gracilaria-Verrucosa

Zhou, YH and Ragan, MA (1995) Characterization of the Polyubiquitin Gene in the Marine Red Alga Gracilaria-Verrucosa. Biochimica Et Biophysica Acta-Gene Structure and Expression, 1261 2: 215-222. doi:10.1016/0167-4781(95)00006-3


Author Zhou, YH
Ragan, MA
Title Characterization of the Polyubiquitin Gene in the Marine Red Alga Gracilaria-Verrucosa
Journal name Biochimica Et Biophysica Acta-Gene Structure and Expression   Check publisher's open access policy
ISSN 0167-4781
Publication date 1995-04-01
Year available 1995
Sub-type Article (original research)
DOI 10.1016/0167-4781(95)00006-3
Open Access Status Not yet assessed
Volume 1261
Issue 2
Start page 215
End page 222
Total pages 8
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Abstract We have cloned a nuclear gene (UB16R) and corresponding cDNAs that encode polyubiquitin in the florideophycidean red alga Gracilaria verrucosa. The gene encodes a polyubiquitin composed of six tandem ubiquitin units, followed by a single glutamine residue. The deduced amino acid sequences are identical among all six units, and identical to the ubiquitin of the florideophyte Aglaothamnion neglectum. There is high sequence similarity among the red algal ubiquitins and those of animals, green plants, fungi and several protists. Only one polyubiquitin gene was found by Southern hybridization analysis of G. verrucosa nuclear DNA. The upstream region of the gene is rich in putative cis-acting transcription-regulatory elements, including a putative heat-responsive element. Poly(A) addition to UB16R mRNA was observed in cDNAs at four different sites, implicating the sequences AATAAA and (or) AGTAAA as poly(A) addition signals. The polyubiquitin genes of red algae show features of concerted evolution, but appear to be subject to less sequence homogenization than those of animals.
Keyword Polyubiquitin Gene
Cdna
Deduced Amino Acid Sequence
(Marine Red Alga)
(Gracilaria-Verrucosa)
Dna-Binding Proteins
Ubiquitin Genes
Heat-Shock
Mammalian-Cells
Expression
Transcription
Organization
Degradation
Sequence
Dictyostelium
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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