The nuclear gene and cDNAs encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa: Cloning, characterization and phylogenetic analysis

Zhou Y.-H. and Ragan M.A. (1995) The nuclear gene and cDNAs encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa: Cloning, characterization and phylogenetic analysis. Current Genetics, 28 4: 324-332. doi:10.1007/BF00326430


Author Zhou Y.-H.
Ragan M.A.
Title The nuclear gene and cDNAs encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa: Cloning, characterization and phylogenetic analysis
Journal name Current Genetics   Check publisher's open access policy
ISSN 0172-8083
Publication date 1995-01-01
Year available 1995
Sub-type Article (original research)
DOI 10.1007/BF00326430
Open Access Status Not yet assessed
Volume 28
Issue 4
Start page 324
End page 332
Total pages 9
Place of publication NEW YORK
Publisher SPRINGER VERLAG
Language eng
Subject 1311 Genetics
Abstract We have cloned and sequenced the single-copy nuclear gene (GapC) encoding the complete 335-amino acid cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC) from the red alga Gracilaria verrucosa. The proline residue which contributes to the specificity of NAD(+) binding in other GAPC-like proteins is present. Putative regulatory regions, including GC-rich regions, a GATA element, and 11-base T- and T/G-clusters, but excluding TATA- and CCAAT-boxes, were identified upstream. Two types of GapC cDNAs differing in polyadenylation site were characterized. An 80-bp phase-two spliceosomal intron was identified in a novel position interrupting the highly conserved cofactor-coding region I. The G. verrucosa GAPC was easily aligned with other known GAPC-type sequences. Inferred phylogenetic trees place red algae among the eukaryote crown taxa, although with modest bootstrap support and without stable resolution among related GAPC lineages.
Keyword Glyceraldehyde-3-phosphate dehydrogenase
Gracilaria verrucosa
Molecular phylogeny of eukaryotes
Spliceosomal intron
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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