Characterization of the Nuclear Gene Encoding Mitochondrial Aconitase in the Marine Red Alga Gracilaria-Verrucosa

Zhou, YH and Ragan, MA (1995) Characterization of the Nuclear Gene Encoding Mitochondrial Aconitase in the Marine Red Alga Gracilaria-Verrucosa. Plant Molecular Biology, 28 4: 635-646. doi:10.1007/BF00021189


Author Zhou, YH
Ragan, MA
Title Characterization of the Nuclear Gene Encoding Mitochondrial Aconitase in the Marine Red Alga Gracilaria-Verrucosa
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
Publication date 1995-07-01
Year available 1995
Sub-type Article (original research)
DOI 10.1007/BF00021189
Open Access Status Not yet assessed
Volume 28
Issue 4
Start page 635
End page 646
Total pages 12
Place of publication DORDRECHT
Publisher Kluwer Academic Publishers
Language eng
Abstract We have cloned a nuclear gene from the marine red alga Gracilaria verrucosa that encodes the complete 779 amino-acid mitochondral aconitase (m-ACN), the first characterized from a photosynthetic organism. The N-terminal 28 deduced amino acids are predicted to constitute the mitochondrial transit peptide, the first described from a red alga. Putative transcriptional cis-acting elements were identified in the upstream untranslated region. The G. verrucosa m-ACN gene (m-ACN) is present in a single copy and is located ca. 1.5 kb upstream from the single-copy polyubiquitin gene. The single spliceosomal intron is located near the 5' end of the region encoding the mature m-ACN in precisely the same location and phase as intron 2 in Caenorhabditis elegans m-ACN; sequences at its 3' and 5' splice junctions and at the predicted lariat branch point conform well to the eukaryote consensus sequences. Multiple protein-sequence alignment of m-ACN, bacterial aconitase (b-ACN) and iron-responsive element-binding protein (IRE-BP), and phylogenetic analyses, revealed that m-ACN does not share a recent common ancestry with either b-ACN or IRE-BP.
Keyword Aconitase
Nuclear-Encoded Mitochondrial Protein
Iron-Responsive Element-Binding Protein
Rhodophyta
Gracilaria Verrucosa
Iron-Responsive-Element
Rna-Binding Protein
Ferritin Messenger-Rna
Sulfur Clusters
Glyceraldehyde-3-Phosphate Dehydrogenase
Functional Implications
Activated Aconitase
Nucleotide-Sequence
Secondary Structure
Molecular-Cloning
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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