Cloning and characterization of a nuclear gene encoding a starch branching enzyme from the marine red alga Gracilaria gracilis

Lluisma A.O. and Ragan M.A. (1998) Cloning and characterization of a nuclear gene encoding a starch branching enzyme from the marine red alga Gracilaria gracilis. Current Genetics, 34 2: 105-111. doi:10.1007/s002940050373


Author Lluisma A.O.
Ragan M.A.
Title Cloning and characterization of a nuclear gene encoding a starch branching enzyme from the marine red alga Gracilaria gracilis
Journal name Current Genetics   Check publisher's open access policy
ISSN 0172-8083
Publication date 1998-01-01
Year available 1998
Sub-type Article (original research)
DOI 10.1007/s002940050373
Open Access Status Not yet assessed
Volume 34
Issue 2
Start page 105
End page 111
Total pages 7
Place of publication NEW YORK
Publisher SPRINGER VERLAG
Language eng
Subject 1311 Genetics
Abstract The biosynthesis of starch in red algae occurs in the cytosol, in contrast to green plants where it takes place in the plastid. We have cloned a nuclear gene from the red alga Gracilaria gracilis that encodes a homolog of starch-branching enzymes (SBEs); this gene, which is apparently intron-free, was designated as GgSBE1. A potential TATA box, CAAT boxes, and other potential regula tory elements were observed in its 5' flanking region. The encoded 766-aa peptide shares significant sequence similarity with SBEs from green plants (at least 40%), and with glycogen-branching enzymes (GBEs) from human (46%) and Saccharomyces cerevisiae (45%). Southern-hybridization analysis indicates that the gene is single-copy, although weaker signals suggest that related genes exist in the genome of G. gracilis. Phylogenetic analyses indicate that GgSBE1 groups within the eukaryote branching enzymes (BEs) and not with eubacterial GBEs, suggesting that its gene has not been derived directly from an endosymbiotic cyanobacterium, but instead is ancestrally eukaryotic.
Keyword Branching enzyme
Floridean starch
Gracilaria
Rhodophyta
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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