The lobster parasite Anophryoides haemophila (Scuticociliatida: Orchitophryidae): Nuclear 18S rDNA sequence, phylogeny and detection using oligonucleotide primers

Ragan, MA, Cawthorn, RJ, Despres, B, Murphy, CA, Singh, RK, Loughlin, MB and Bayer, RC (1996) The lobster parasite Anophryoides haemophila (Scuticociliatida: Orchitophryidae): Nuclear 18S rDNA sequence, phylogeny and detection using oligonucleotide primers. Journal of Eukaryotic Microbiology, 43 4: 341-346. doi:10.1111/j.1550-7408.1996.tb03998.x


Author Ragan, MA
Cawthorn, RJ
Despres, B
Murphy, CA
Singh, RK
Loughlin, MB
Bayer, RC
Title The lobster parasite Anophryoides haemophila (Scuticociliatida: Orchitophryidae): Nuclear 18S rDNA sequence, phylogeny and detection using oligonucleotide primers
Journal name Journal of Eukaryotic Microbiology   Check publisher's open access policy
ISSN 1066-5234
Publication date 1996-07-01
Year available 1996
Sub-type Article (original research)
DOI 10.1111/j.1550-7408.1996.tb03998.x
Open Access Status Not yet assessed
Volume 43
Issue 4
Start page 341
End page 346
Total pages 6
Place of publication LAWRENCE
Publisher SOC PROTOZOOLOGISTS
Language eng
Abstract We have determined the nucleotide sequence of the nuclear gene encoding small-subunit ribosomal ribonucleic acid of the ciliate Anophryoides haemophila, a parasite of the American lobster Homarus americanus. The gene is 1763 bp in length, and has a guanosine-plus-cytosine content of 43.9%. Inferred phylogenetic frameworks strongly support the monophyly of the scuticociliates, and suggest that order Scuticociliatida should be elevated to at least subclass rank. Oligonucleotide probes based on A. haemophila ssu-rDNA can discriminate between DNAs of A. haemophila and other investigated hymenostome ciliates, and effectively prime polymerase chain reaction-based detection of A. haemophila deoxyribonucleic acid against at least a 1600-fold excess of total deoxyribonucleic acid from H. americanus.
Keyword bumper-car disease
ciliate systematics
Kishino-Hasegawa test
PCR-based detection
rRNA gene phylogeny
Templeton-Felsenstein test
Ribosomal-Rna Gene
Phylum Ciliophora
Molecular Phylogeny
Plasmid Dna
Identification
Ciliate
Evolution
Origins
Probes
Tree
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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