cDNA cloning and characterization of the nuclear gene encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa

Zhou, Yi-Hong and Ragan, Mark A. (1993) cDNA cloning and characterization of the nuclear gene encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa. Current Genetics, 23 5-6: 483-489. doi:10.1007/BF00312639


Author Zhou, Yi-Hong
Ragan, Mark A.
Title cDNA cloning and characterization of the nuclear gene encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa
Formatted title
cDNA cloning and characterization of the nuclear gene encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa
Journal name Current Genetics   Check publisher's open access policy
ISSN 0172-8083
Publication date 1993-05-01
Year available 1993
Sub-type Article (original research)
DOI 10.1007/BF00312639
Open Access Status Not yet assessed
Volume 23
Issue 5-6
Start page 483
End page 489
Total pages 7
Place of publication NEW YORK
Publisher Springer-Verlag
Language eng
Abstract Using a PCR-generated homologous probe, we have recovered a cDNA (GapA cDNA) encoding the complete 338 amino-acid chloroplast GAPDH of the marine red alga Gracilaria verrucosa, together with its 78 amino-acid transit peptide. This cDNA was readily aligned with chloroplast-localized GAPDH genes (GapA and GapB) of green plants. The proline residue which contributes to the specificity of NAD+ binding to cytosolic GAPDHs is absent from the deduced polypeptide chain of G. verrucosa GapA as is also the case in the chloroplast GAPDHs of plants. The transit peptide shows a high proportion of random coil, an amino-terminal Met-Ala dipeptide, a high content of hydroxylamino acids, and a net positive charge. The polyadenylation signal appears to be AGTAAA. Genomic Southern-hybridization data indicate that only one chloroplast-GAPDH gene may occur in G. verrucosa. Bootstrapped parsimony trees indicate that the G. verrucosa GapA gene is a sister group to plant chloroplast-GAPDH genes, and are most readily interpreted as showing that red algal and plant chloroplast-localized GAPDHs arose in a single endosymbiotic event.
Keyword Glyceraldehyde-3-Phosphate Dehydrogenase Cdna
Chloroplast Isoenzyme
Gracilaria-Verrucosa
Molecular Evolution
Sequence-Analysis
Nucleotide-Sequence
Plastid Genome
Origin
Evolution
Precursors
Transport
Promoter
Proteins
Region
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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